Nf2 (NM_001252251) Mouse Untagged Clone

CAT#: MC228395

Nf2 (untagged) - Mouse neurofibromatosis 2 (Nf2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nf2
• Synonyms: merlin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228395 representing NM_001252251
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGCCATCGCTTCTCGCATGAGCTTCAGCTCACTCAAGAGGAAGCAGCCCAAGACATTCACGG
  TGCGGATCGTCACCATGGACGCCGAGATGGAGTTCAACTGCGAGATGAAATGGAAGGGGAAGGACCTGTT
  TGATTTGGTGTGCCGGACACTGGGGCTTCGGGAAACCTGGTTCTTTGGACTGCAGTATACAATCAAGGAC
  ACGGTGGCCTGGCTCAAAATGGACAAGAAGGTGTTGGATCATGATGTTTCGAAGGAAGAACCAGTTACCT
  TTCACTTCCTGGCCAAATTTTATCCTGAAAATGCTGAGGAGGAGCTAGTTCAAGAGATCACGCAACACTT
  ATTTTTCTTACAGGTGAAGAAGCAGATTTTGGATGAAAAGGTCTACTGCCCTCCCGAGGCGTCCGTGCTC
  TTGGCGTCATATGCTGTCCAGGCCAAGTATGGCGACTATGACCCCTCTGTGCACAAGCGGGGATTTTTAG
  CCCAAGAGGAATTGCTCCCGAAAAGGGTGATAAATCTCTATCAGATGACTCCGGAAATGTGGGAGGAGAG
  AATTACGGCTTGGTATGCGGAGCACCGGGGCAGAGCCAGGGATGAAGCTGAAATGGAGTATTTGAAGATA
  GCTCAGGACCTGGAGATGTATGGTGTGAACTACTTTACAATCCGGAATAAAAAGGGCACAGAGTTGCTGC
  TTGGAGTGGATGCTCTTGGGCTTCATATCTATGACCCTGAGAACAGGCTGACCCCCAAGATCTCCTTCCC
  ATGGAATGAAATCCGAAACATCTCCTACAGCGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATT
  GATGTCTTCAAATTTAACTCCTCAAAGCTTCGTGTTAATAAGCTGATTCTTCAGCTATGTATTGGGAACC
  ATGACCTATTTATGAGGCGACGGAAAGCTGACTCTTTAGAAGTTCAGCAGATGAAAGCCCAGGCCAGGGA
  AGAGAAGGCTAGAAAGCAGATGGAAAGGCAGCGGCTGGCTCGAGAGAAGCAGATGCGGGAGGAGGCCGAG
  CGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGAAGAAGCAACGATGGCCAATGAAGCTC
  TGATGCGCTCTGAGGAGACAGCTGATCTGTTGGCTGAAAAGGCTCAGATCACAGAGGAGGAGGCCAAGCT
  TTTGGCACAAAAGGCTGCAGAGGCTGAGCAAGAGATGCAGCGAATCAAGGCCACGGCCATTCGGACAGAG
  GAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAGGCTGAAGTGCTGGCATTGAAGATGGCTGAGGAGT
  CAGAGAGGAGGGCCAAGGAGGCTGATCAGTTAAAGCAAGACTTGCAAGAAGCCAGAGAAGCAGAGCGAAG
  AGCCAAGCAGAAGCTCTTAGAAATCGCCACCAAGCCCACCTATCCACCCATGAACCCAATTCCACCACCA
  CTGCCTCCTGACATACCGAGCTTCGACATTATTGCTGACAGCTTGTCATTTGACTTCAAGGATACGGACA
  TGAAGCGACTTTCCATGGAGATAGAGAAAGAAAAAGTGGAGTACATGGAGAAGAGCAAGCACCTGCAGGA
  GCAGCTCAACGAGCTCAAGACGGAGATCGAGGCCTTGAAACTCAAAGAGCGGGAGACGGCCTTGGACGTC
  CTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCATAATACCATTAAAAAGCCTCAAGCCC
  AAGGCAGAAGACCTATCTGCATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252251
• Insert Size: 1776 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252251.1, NP_001239180.1
• RefSeq Size: 2649 bp
• RefSeq ORF: 1776 bp
• Locus ID: 18016
• UniProt ID: P46662
• Cytogenetics: 11 3.0 cM
• Gene Summary: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.