Adam17 (NM_001291871) Mouse Untagged Clone

CAT#: MC228390

Adam17 (untagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam17
• Synonyms: CD156b; Tac; Tace
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228390 representing NM_001291871
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAATAGAGCTAATTGACCGAGTTGATGACATAT
  ACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAGCAGATTCGAATTCT
  CAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTTTCCCAAACGAAGAG
  AAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGAAGAAGCATCTAAAG
  TCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTAGCTTACGTTGGTTC
  TCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACATC
  TATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAAGGAAGCTGACCTGG
  TTACAACTCATGAATTGGGACATAATTTTGGAGCAGAACATGACCCTGATGGGCTAGCAGAATGTGCCCC
  AAATGAGGACCAAGGAGGAAAGTATGTCATGTATCCCATAGCTGTGAGCGGTGACCACGAGAATAATAAG
  ATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGTAAGGCTCAAGAGTGCTTCCAGG
  AGCGCAGCAACAAGGTGTGTGGCAACTCCAGGGTGGATGAAGGAGAGGAGTGTGACCCGGGTATTATGTA
  CCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCGGGTGTGCAGTGCAGTGATAGGAAC
  AGTCCTTGCTGTAAAAACTGTCAGTTTGAGACGGCGCAGAAGAAGTGCCAGGAGGCTATTAATGCTACAT
  GCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCCGGAGATGCTGAAGATGACAC
  TGTGTGCTTGGACCTTGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGAGGGAGCAGGAGCTG
  GAGTCCTGCGCATGCGTTGACACTGACAACTCGTGCAAGGTGTGCTGCAGGAACCTTTCTGGCCCGTGTG
  TGCCGTACGTCGATGCAGAGCAAAAGAACTTGTTTTTGAGGAAAGGGAAGCCATGTACAGTAGGGTTTTG
  CGACATGAATGGCAAATGTGAGAAACGAGTACAGGACGTAATTGAGCGATTTTGGGATTTCATTGACCAG
  CTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGATAACATCGTTGGGTCTGTTCTGGTTTTCTCCTTGA
  TATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAGCAGTATGAATCCCT
  GTCTCTGTTTCATCACAGTAACATTGAGATGCTGAGCAGCATGGACTCAGCATCTGTTCGCATCATCAAG
  CCCTTTCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCAGCTGCCATGATGCCGCCAGTAC
  CTGCAGCTCCAAAACTGGACCACCAGAGGATGGACACCATCCAGGAAGACCCCAGCACAGACTCACATGC
  AGATGATGACGGTTTTGAGAAGGACCCCTTCCCCAACAGCAGCACAGCTGCCAAGTCCTTTGAGGATCTC
  ACAGACCACCCAGTCACCAGGAGCGAAAAGGCGGCCTCATTCAAGCTGCAGCGTCAGAGCCGAGTTGACA
  GCAAAGAGACAGAGTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291871
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291871.1, NP_001278800.1
• RefSeq Size: 4470 bp
• RefSeq ORF: 1770 bp
• Locus ID: 11491
• UniProt ID: Q9Z0F8
• Cytogenetics: 12 8.3 cM
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero or fail to survive beyond one week of age. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]
  Transcript Variant: This variant (4) uses an alternate 5' splice site, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 4) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.