Fzd5 (NM_001042659) Mouse Untagged Clone

CAT#: MC228382

Fzd5 (untagged) - Mouse frizzled homolog 5 (Drosophila) (Fzd5), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fzd5
• Synonyms: 5330434N09Rik; AI427138; Fz-5; Fz5; mFz5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228382 representing NM_001042659
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGACCCGACCCGTCTGCGCCTCCCTCTCTTCTGCTGCTGTTGCTGGCGCAGCTGGTGGGCCGGG
  CAGCGGCCGCCTCCAAGGCCCCGGTGTGCCAGGAAATCACGGTGCCCATGTGCCGAGGCATCGGCTACAA
  CCTGACGCACATGCCCAACCAGTTCAACCATGACACGCAGGACGAAGCAGGCCTGGAGGTGCACCAATTC
  TGGCCGCTTGTGGAGATCCACTGCTCACCGGACCTGCGCTTCTTCCTGTGCTCTATGTACACGCCCATCT
  GTTTGCCTGACTACCACAAGCCGCTACCACCGTGCCGTTCCGTGTGCGAGCGCGCCAAGGCCGGCTGCTC
  GCCGCTCATGCGCCAGTACGGCTTCGCCTGGCCCGAGCGCATGAGCTGCGACCGCCTCCCTGTGCTGGGC
  GGCGACGCCGAGGTTCTGTGTATGGATTATAACCGAAGCGAAGCCACCACCGCGTCCCCTAAGTCCTTCC
  CGGCCAAACCTACACTCCCAGGACCACCAGGGGCGCCATCTTCCGGGGGCGAGTGCCCCTCGGGAGGCCC
  ATCCGTGTGCACGTGCCGCGAGCCCTTCGTGCCCATCCTGAAGGAGTCACACCCACTCTACAACAAGGTG
  CGCACCGGCCAAGTGCCCAACTGCGCGGTGCCCTGCTACCAGCCGTCCTTCAGCCCGGACGAGCGCACAT
  TCGCCACCTTCTGGATTGGCCTGTGGTCTGTGCTGTGCTTCATCTCCACGTCCACCACCGTTGCCACCTT
  CCTCATTGACATGGAACGATTCCGCTACCCTGAGCGCCCCATCATCTTCTTGTCTGCGTGCTACCTGTGT
  GTGTCACTGGGATTCTTGGTGCGCCTGGTAGTGGGCCATGCCAGCGTCGCTTGCAGCCGTGAGCACAGCC
  ACATTCACTATGAGACTACCGGCCCTGCGCTGTGCACGGTTGTCTTCCTCTTAGTCTATTTCTTTGGCAT
  GGCCAGCTCCATCTGGTGGGTCATCCTGTCGCTCACCTGGTTCTTGGCGGCTGGCATGAAGTGGGGCAAT
  GAAGCCATCGCAGGTTATGCACAGTACTTCCACCTTGCTGCCTGGCTCATCCCCAGTGTCAAGTCCATTA
  CGGCGCTGGCACTGAGCTCGGTGGACGGGGACCCAGTGGCTGGCATCTGCTATGTGGGCAACCAAAACCT
  GAACTCACTACGAGGCTTTGTCTTGGGCCCACTGGTGCTGTACCTGTTGGTGGGCACGCTCTTCCTTCTG
  GCAGGCTTCGTGTCACTCTTCCGCATCCGGAGCGTCATCAAGCAGGGTGGCACTAAGACGGACAAGCTAG
  AGAAGCTCATGATCCGCATCGGCATCTTCACCCTGCTCTACACGGTGCCAGCCAGCATCGTGGTGGCCTG
  CTACCTGTATGAGCAGCACTACCGGGAGAGCTGGGAGGCAGCCCTCACCTGCGCGTGTCCGGGACCGGAC
  GCTGGCCAGCCACGCGCCAAACCCGAGTACTGGGTGCTCATGCTCAAGTACTTCATGTGCCTGGTGGTGG
  GCATCACGTCGGGAGTCTGGATCTGGTCCGGCAAGACTCTGGAGTCTTGGCGGCGGTTCACCAGCCGCTG
  CTGCTGCAGCTCTCGGCGGGGCCACAAGAGCGGTGGCGCTATGGCCGCAGGAGACTATGCGGAGGCCAGC
  GCCGCGCTCACCGGCAGGACCGGGCCGCCTGGCCCCACCGCCGCATACCACAAGCAAGTGTCCCTGTCGC
  ACGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042659
• Insert Size: 1758 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042659.1, NP_001036124.1
• RefSeq Size: 6823 bp
• RefSeq ORF: 1758 bp
• Locus ID: 14367
• UniProt ID: Q9EQD0
• Cytogenetics: 1 32.74 cM
• Gene Summary: Receptor for Wnt proteins (PubMed:11092808, PubMed:18230341). Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (PubMed:11092808). In neurons, activation of WNT7A promotes formation of synapses (By similarity). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:18230341). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (PubMed:11092808).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.