Nprl3 (NM_001284359) Mouse Untagged Clone

CAT#: MC228373

Nprl3 (untagged) - Mouse nitrogen permease regulator-like 3 (Nprl3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nprl3
• Synonyms: Aag; CGTHBA; HS-26; HS-40; m(alpha)RE; Mare; Phg; Prox1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228373 representing NM_001284359
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACAATACCAGCCCCATCAGCGTGATCCTGGTGAGCTCGGGCAGCAGGGGCAATAAGCTGCTCT
  TCAGGTACCCCTTCCAGAGGAGCCAGGAGCACCCCGCGTCCCAGACAAATAAACCACGGAGCAGATATGC
  TGTCAACAACACTGGCGAACATGCTGATGACCAGGACGGTGACTCCAGCAAGCCATGTCCTCCAACCGAT
  GAGCAATTGGTTGCAGGGTTCTCGGATGTTATTCTGGCAACAATTTTGGCAACCAAATCTGAAATGTGCG
  GCCAAAAATTTGAACTGAAGATTGATAATGTACGGTTTGTTGGGCACCCAACACTGCTGCAGCATGCTCT
  GGGGCAGGTCTCCAAAACAGATCCATCTCCAAAGCGGGAAGCTCCGACCATGATTCTTTTTAACGTGGTA
  TTTGCACTGAGGGCCAATGCTGATCCATCAGTGATAAACTGTCTACACAACTTGTCCCGCCGAATTGCTA
  CTGTCCTGCAGCATGAGGAGCGCCGCTGCCAGTACCTCACTCGAGAGGCCAAGCTGATCCTAGCACTTCA
  GGATGAGGTGTCTGCTATGGCTGATGCAAATGAAGGTCCTCAGTCCCCATTCCAGCACATCCTACCCAAG
  TGCAAGCTGGCTCGGGACCTCAAAGAAGCTTATGACAGCCTGTGCACATCTGGTGTGGTACGGCTTCACA
  TTAACAGCTGGCTGGAAGTGAGTTTCTGTCTGCCCCACAAGATCCACTATGCAGCTTCAAGTCTGATTCC
  TCCTGAGGCTATTGAACGGAGCCTGAAAGCCATCCGCCCGTACCATGCCTTGCTACTTCTCAGTGACGAG
  AAGTCTCTGCTGAGTGAGCTTCCCATTGACTGCTCCCCGGCTCTGGTGCGGGTGATCAAGACCACGTCTG
  CTGTGAAGAACCTGCAGCAGCTAGCCCAGGATGCCGATCTGGCCTTGCTGCAGGTCTTCCAGTTGGCAGC
  TCACCTGGTATACTGGGGCAAGGCTGTCATCATCTACCCACTGTGTGAGAACAACGTCTATGTCATGTCT
  CCCAATGCCAGTGTGTGTCTGTACTCCCCGCTAGCTGAACAGTTCTCCCGGCAGTTCCCGTCCCATGACC
  TGCCATCTGTCCTGGCCAAGTTTTCCTTGCCTGTTTCTTTATCGGAGTTCAGGAGCCCTCTGGCTCCCCC
  TGCACAGGAGACCCAGCTCATCCAGATGGTGGTGTGGATGCTGCAGCGCCGGCTCCTCATCCAGCTGCAT
  ACCTATGTTTGCCTAATGGCCTCACCCAGCGAAGAGGAGCCCCGACTGCGAGAGGATGATGTCCCCTTCA
  CAGCCAGAGTTGGTGGCCGCAGCCTCAGCACACCCAATGCTCTAAGCTTTGGCTCCCCAACCAGCAGTGA
  CGACATGACCCTTACCAGCCCCAGTATGGACAACTCAAGTGCTGAGCTGCTCCCCAGTGGGGACTCACCA
  CTGAACAAGAGGATGACAGAGAACCTGCTGGCTAGCCTCTCAGAGCATGAGCGGGCTGCTATCCTCAATG
  TGCCTGCAGCCCAAAACCCTGAGGACCTCCGCATGTTTGCCAGGCTCCTTCACTACTTCCGTGGCCGCCA
  CCATCTGGAGGAGATCATGTACAATGAGAACACTCGGCGCTCCCAGCTACTCATGCTCTTTGACAAATTC
  CGCAGTGTGCTGGTGGTGACCACCCATGAGGACCCTGTTATTGCCGTCTTCCAGGCACTGCTCACATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284359
• Insert Size: 1749 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284359.1, NP_001271288.1
• RefSeq Size: 2904 bp
• RefSeq ORF: 1749 bp
• Locus ID: 17168
• UniProt ID: Q8VIJ8
• Cytogenetics: 11 18.83 cM
• Gene Summary: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).