Acsm3 (NM_016870) Mouse Untagged Clone

CAT#: MC228361

Acsm3 (untagged) - Mouse acyl-CoA synthetase medium-chain family member 3 (Acsm3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acsm3
• Synonyms: Sa; Sah
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228361 representing NM_016870
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGTTACTCCGTGCTAGGTGTTTTCAGCGCCTAGCAATTCCTGATCCTATGAGAGTCTTGTATA
  AAGATTACAGAACAGCGACCCCTCAGAACTTTTCCAACTATGAGTCCATGAAACAAGACTTCAAAATAGA
  GATTCCAGAGTATTTCAATTTTGCAAAAGATGTCCTGGACCAATGGACCAATATGGAAAAGGCTGGAAAG
  AGACTTTCCAATCCAGCCTTCTGGTGGATAGATGGGAATGGAGAAGAGCTGAGATGGAGTTTTGAAGAAC
  TTGGGTTGTTATCCAGGAAATTTGCCAACATACTCACAGAAGCCTGCTCCCTGCAAAGAGGAGACAGAGT
  AATGGTGATACTGCCCAAGATCCCAGAGTGGTGGCTTGCAAATGTGGCCTGTCTGCGAACAGGGACAGTT
  TTAATTCCAGGAACCACTCAGCTGACCCAGAAAGACATCCTCTATAGACTACAATCTTCAAAAGCTAAGT
  GCATTATTACCGATGATACTTTGGCCCCAGCAGTAGATGCCGTGGCAGCTAAATGTGAAAATCTCCACTC
  CAAATTAATTGTGTCTCAGCACTCCAGAGAAGGCTGGGGGAACCTCAAGGAGATGATGAAATATGCCAGT
  GACAGCCACACTTGTGTGGACACAAAACACGACGAGATGATGGCCATCTACTTCACCAGTGGGACAACTG
  GGCCTCCTAAGATGATTGGACACACCCACAGCAGCTTTGGTTTAGGATTGTCTGTCAATGGAAGGTTCTG
  GCTGGATTTGATAGCCTCCGATGTGATGTGGAATACTTCAGATACAGGCTGGGCAAAGTCTGCATGGAGT
  AGTGTTTTTTCTCCATGGACCCAAGGAGCATGTGTTTTTGCACACTATTTGCCCCGTTTTGAATCAACTT
  CCATCTTGCAAACCCTCTCCAAGTTCCCCATCACTGTCTTCTGTTCTGCACCAACTGCCTACCGGATGCT
  TGTTCAGAATGACATGAGCAGCTATAAGTTCAACAGTTTGAAGCACTGTGTCAGTGCTGGAGAACCTATT
  AACCCTGAAGTGATGGAACAATGGAGAAAGAAGACGGGCCTAGACATCTATGAAGGATATGGACAGACAG
  AAACGGTGCTGATCTGTGGAAATTTCAAGGGGATGAAAATTAAGCCCGGCTCAATGGGAAAGCCTTCTCC
  TGCTTTTGATGTGAAGATTTTAGATGAAAATGGTGCCACTCTTCCTCCTGGACAAGAAGGGGATATTGCT
  CTTCAAGTTCTTCCTGAGCGACCATTTGGCCTTTTTACTCATTATGTAGATAATCCTTCCAAAACGGCTT
  CAACTCTACGAGGGAGTTTCTACATTACTGGGGACAGAGGATATATGGATGAAGATGGCTATTTCTGGTT
  TGTTGCAAGATCAGATGATATCATATTATCTTCTGGTTACCGAATTGGACCATTTGAGGTAGAAAGTGCC
  CTCATAGAACATCCTTCCATCGCAGAGTCAGCTGTTGTCAGCAGTCCAGACCCCATCAGAGGAGAGGTAG
  TAAAGGCTTTTATTGTCCTGAATCCTGATTACAAGTCCCATGATCAAGAGCAACTGAAAAAGGAGATCCA
  GGAGCACGTGAAGAAGACTACAGCGCCTTACAAATACCCCAGAAAGGTAGAATTTATTGAAGAGCTGCCG
  AAGACTGTCAGTGGGAAGGTCAAAAGAAATGAACTGAGGAAAAAGGAATGGGTAACAACTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016870
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016870.3, NP_058566.3
• RefSeq Size: 2777 bp
• RefSeq ORF: 1743 bp
• Locus ID: 20216
• UniProt ID: Q3UNX5
• Cytogenetics: 7 F2
• Gene Summary: Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcripts. The three transcript variants all encode the same protein.