Pdp1 (NM_001290387) Mouse Untagged Clone

CAT#: MC228358

Pdp1 (untagged) - Mouse pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pdp1
• Synonyms: Gm1024; Ppm2c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228358 representing NM_001290387
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCGGCGGTGCGCCTGTCCGGGCTCCTGGAGTTGCCCGCCGGGGGCTGTGATGCCGCCTCGCC
  TGCCGGGAATCCCAGTCAGAACGTCCAGTCTGCCACTGTTCTCTGATGCCATGCCAGCACCAACTCAACT
  GTTTTTTCCTCTCGTCCGTAACTGTGAACTGAGCAGAATCTATGGCACTGCATGTTACTGCCACCACAAA
  CATCTCTGCTGTTCACCACCATACATTCCTCAGAATCGTCTGAGGTACACACCCCATCCTGCATATGCTA
  CCTTTTGTAGGCCACGGGAGAACTGGTGGCAGTATACTCAAGGAAGGAGATACGCTTCTACACCGCAGAA
  ATTTTACCTCACACCTCCACAAGTCAACAGCATCCTTAAAGCTAATGAATACAGCTTCAAAGTACCAGAA
  TTTGATGGCAAAAATGTCAGTTCCATTCTTGGATTTGACAGCAATCAGCTGCCTGCAAATGCACCCATAG
  AGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTCTTGGGGGTTTTTGATGGTCATGC
  AGGCTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTCTATTATATTGCTGTTTCCTTGTTGCCCCAT
  GAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAGTGGTCGGGCACTGCTACCTATCCTTCAGTGGCACA
  AGCACCCCAATGATTACTTCAGTAAGGAGGCGTCCAAATTGTATTTCAACAGCTTGAGGACTTACTGGCA
  AGAACTCATAGACCTCAATACTGGAGAATCAGCTGATATTGATGTTAAGGAGGCTTTAATTAATGCTTTC
  AAGAGACTCGATAATGACATTTCATTGGAGGCTCAAGTTGGTGATCCTAATTCTTTTCTAAATTACCTGG
  TGCTTCGGGTAGCATTTTCTGGGGCTACTGCTTGTGTGGCCCATGTAGATGGTGTTGACCTCCATGTGGC
  TAACACTGGTGATAGTAGAGCCATGCTAGGTGTGCAAGAAGAAGATGGCTCTTGGTCAGCAGTCACACTC
  TCTAATGACCACAATGCTCAGAATGAAAGAGAACTAGAGCGTCTGAAACTGGAACACCCAAAAAATGAGG
  CCAAGAGCGTGGTAAAGCAGGACCGGCTGCTTGGCTTGCTGATGCCCTTTAGGGCTTTTGGAGATGTAAA
  GTTCAAATGGAGCATTGACCTTCAAAAGAGAGTGATAGAGTCTGGCCCAGACCAGTTGAATGACAATGAA
  TACACCAAGTTTATCCCTCCTAACTATCATACACCTCCTTATCTTACTGCTGAGCCAGAGGTAACTTATC
  ACAGATTAAGGCCACAGGATAAATTCCTAGTGTTAGCAACTGATGGGTTGTGGGAGACTATGCATAGACA
  GGATGTGGTTAGGATTGTGGGTGAATACTTAACTGGTATGCATCACCAACAGCCAATAGCAGTTGGTGGG
  TACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAAAGGAGAGCAAAGATGTCATCAGTGTTTG
  AGGATCAGAATGCAGCAACCCATCTCATTCGCCATGCTGTAGGCAATAATGAATTTGGGGCTGTTGATCA
  TGAACGACTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGGATGTATAGAGATGACATTACAATC
  ATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCATACCAAAACCAGGAACAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290387
• Insert Size: 1737 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290387.1, NP_001277316.1
• RefSeq Size: 4149 bp
• RefSeq ORF: 1737 bp
• Locus ID: 381511
• UniProt ID: Q3UV70
• Cytogenetics: 4 A1
• Gene Summary: Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) encodes the longest isoform (d).