Sybu (NM_001285843) Mouse Untagged Clone

CAT#: MC228324

Sybu (untagged) - Mouse syntabulin (syntaxin-interacting) (Sybu), transcript variant g

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sybu
• Synonyms: Golsyn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228324 representing NM_001285843
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTCTATGGAGCTTACTCTCTTATTACTCATAATGTGTGGTTGGCCCAAATGGCAGAAGGCTCCA
  CCCCCGAGAGCATGAGTGTACCGGTGACTGTTATGTTTCCAACTTGTCACATGGGTCAAGTTTTAGGCAG
  TGAAGCAGATTTTAGCTCCTCGAGCAGCACAGGCAGCATCTCGGCTCCTGAGGTCCACATGTCCACGACA
  GGAAACAAGAGAGCCTCTTTCTCACGCAACCGAGGCCCTCATGGGCGGAGCAATGGAGCATCATCCCACA
  AGTCTGGCAGCAGCCCACCATCCCCAAGGGAAAAAGACCTTGTGTCTATGCTGTGCAGGAATCCACTGAG
  CCCCAGTAACATCCACCCTAGTTACGCCCCTTCTTCTCCAAGTAGCAGCAACTCCGGCTCCTACAAAGGA
  AGTGATTGTAGTCCAGTCATGAGAAGGTCTGGAAGATACATGTCTTGTGGCGAAAATCACGGTGTCAAAC
  CCCCAAATCCAGAACAGTATTTGACTCCTCTGCAGCAGAAGGAGGTCACAGTGAGGCACCTGAGGACCAA
  GCTGAAGGAGTCTGAGCGCCGACTCCATGAGAGGGAATCTGAAATCATGGAGCTCAAGTCTCAGCTGGCT
  CGAATGAGGGAGGACTGGATTGAGGAAGAGTGCCACAGAGTGGAGGCTCAGTTGGCACTCAAAGAAGCCA
  GAAAAGAGATTAAACAGCTCAAACAGGTCATCGAGACTATGAGGAGCAGCTTGGCTGATAAAGATAAAGG
  CATTCAGAAGTACTTTGTGGACATAAACATCCAAAACAAGAAACTGGAGTCTCTGCTTCAAAGCATGGAG
  ATGGCACACAATAGTTCCCTGAGGGATGAACTGTGTCTTGACTTTTCCTTTGATTCCCCAGAGAAAAGTT
  TACCCCTGAGCAGCACATTTGACAAGTTGCCAGATGGGTTATCTCTGGAAGAACAGATAACAGAGGAAGG
  TGCTGACAGTGAGCTTCTGGTGGGAGACAGCATGGCTGAAGGCACAGATCTGTTAGATGAGATGGTGACT
  GCCACCACCACAGAATCCAGTGGCCTGGAGTTTGTTCATTCCACTCCAGGGCCACAAGCCCTCAAGGCTC
  TCCCCTTGGTGAGCCACGAAGAGGGCATTGCGGTGATGGAGCAAGCCGTGCAGACCGACGTGGTGCCGTT
  CAGCCCTGCCATCTCAGAGCTCATTCAGAGTGTGCTAAAGCTGCAGGACTACTGTCCCACAAGCTCAGCA
  TCTCCAGATGAATCTGGAGCTGACTCGATGGAAAGCTTCTCAGAATCTATCTCTGCCTTAATGCTTGATT
  TAACTCCAAGAAGTCCTAACTCGGCCATCCTTCTGTCTCCTGTGGAGATCCCATTCAGCAAGGGGGCTAT
  GGAAGCCCATGCAAATCGCCTCATGAGAGAGCTAGATTTTGCAGCCTACACAGAAGAAAGATTGGACAGT
  GTCCTCTCACTGTCCCAGGGCAGTGTTGTGAGGCAGTACTGGAGCAGCAATTTCTTGGTGGATCTACTGG
  CTGTGGCTGCCCCTGTGGTACCCACTGTTCTGTGGGCATTCAGTACTCAGAGAGGGGGTACAGATCCTGT
  CTACAACATCGGAGCTTTGCTCCGAGGTTGCTGTGTGGTGGCCCTACACTCACTACGCCGCACCGCTTTC
  CACATGAAAACCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285843
• Insert Size: 1695 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285843.1, NP_001272772.1
• RefSeq Size: 2506 bp
• RefSeq ORF: 1695 bp
• Locus ID: 319613
• UniProt ID: Q8BHS8
• Cytogenetics: 15 B3.2
• Gene Summary: Part of a kinesin motor-adapter complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (g) has multiple differences, compared to variant 1. These differences result in a distinct 5'UTR and cause translation initiation at an alternate start codon. The encoded isoform (e) has a distinct and shorter N-terminus, compared to isoform a.