Cpeb1 (NM_001252525) Mouse Untagged Clone

CAT#: MC228318

Cpeb1 (untagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpeb1
• Synonyms: AU024112; Cpe-bp1; Cpeb; mCPEB; mCpeb-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228318 representing NM_001252525
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCTCTCTGGAAGAAGCAGCAGGAAGAATAAAGGATTGCTGGGACAACCAAGAAGTTCCTGCAC
  TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG
  TAAAGTCTGTACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGCAGACTTCCAGGACTCTGAA
  GAAACAGTTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGTGGCCTCCCAGATGCAA
  ATGGCTTGTGCCTTGGCCTGCAGTCCCTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTC
  AGACTCCTCAGCCCAGAGCAGCACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTA
  GGAAAAGCCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCACCCT
  CAGTTAGAGGATCTCGCCTGGACACCCGGCCCATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGA
  CACGAGTGGCTTCAGCTCTGGATCAGACCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCT
  CTGCCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGCGTTGGGTCCCGAATGG
  ACCAAGAACAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAGC
  TTCTGTGTGGCCATCTTGGGACCTTCTTGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGA
  CTCCACCGGCAGGCTGCAGCTGTGAATGAAGCTACGTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACT
  ATAAGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGCGTTCCTTGGGATATTACAGAAGCTGGATT
  GGTTAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGG
  TGTCCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGG
  CCTTGCTTCAGGCTTGTTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTC
  CAGCCGAAGGATGCGCTGCAAAGAGGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGG
  AGCCCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTG
  AGGCTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCA
  CAAGTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTATCTGAAAGCAGTCACCGCT
  GCTTTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATTGACCCCTATCTAGAAGATTCTC
  TGTGTCTTATCTGCAGCTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTG
  CAGAAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCATAGCCCCCTAATGAGGAACCAG
  AAGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252525
• Insert Size: 1689 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252525.1, NP_001239454.1
• RefSeq Size: 3141 bp
• RefSeq ORF: 1689 bp
• Locus ID: 12877
• UniProt ID: P70166
• Cytogenetics: 7 D3
• Gene Summary: Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress (By similarity). Required for cell cycle progression, specifically for prophase entry (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).