Add2 (NM_001271860) Mouse Untagged Clone

CAT#: MC228316

Add2 (untagged) - Mouse adducin 2 (beta) (Add2), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Add2
• Synonyms: 2900072M03Rik; add97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228316 representing NM_001271860
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAGGACACGGTTCCCGAGGCGGCCTCCCCGCCACCCTCTCAGGGGCAGCACTACTTTGACCGGT
  TCTCTGAAGATGATCCTGAGTACTTGCGACTTCGCAACCGTGCAGCTGACCTGCGACAGGACTTCAACTT
  GATGGAGCAGAAGAAGAGGGTCACCATGATCCTGCAGAGCCCTTCTTTCAGGGAGGAGCTGGAAGGCCTC
  ATCCAGGAGCAAATGAAGAAGGGCAACAACTCCTCCAACATCTGGGCCCTCCGACAGATCGCGGACTTCA
  TGGCCAGCACCTCCCACGCAGTCTTCCCAGCTTCTTCCATGAACTTCTCCATGATGACACCCATCAACGA
  CCTCCACACTGCCGACTCCCTGAACCTGGCCAAGGGGGAGAGGCTTATGCGGTGCAAGATCAGCAGCGTC
  TACCGTCTCCTGGACCTCTACGGCTGGGCGCAGCTCAGTGACACCTACGTCACGCTGAGAGTGAGCAAGG
  AGCAGGACCACTTCCTGATCAGCCCCAAGGGGGTTTCCTGCAGCGAGGTCACAGCCTCCAGCCTGATTAA
  AGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGTTGCTTCCCGGTGGACACCACGGGCTTCAGT
  CTGCACTCAGCCATCTATGCCGCCAGGCCCGACGTGCGGTGTGCCATCCACCTGCACACGCCTGCCACCG
  CAGCGGTGTCAGCTATGAAGTGCGGCCTCCTGCCGGTCTCCCATAATGCCCTGCTGGTGGGGGACATGGC
  CTACTATGACTTCAATGGGGAAATGGAGCAGGAAGCTGACCGAATCAACTTGCAGAAGTGCCTTGGACCC
  ACCTGCAAGATTCTGGTGCTAAGAAACCATGGCATGGTCGCCCTGGGTGACACCGTGGAGGAAGCTTTCT
  ACAAGGTCTTCCATCTGCAGGCTGCGTGTGAGGTACAGGTGTCGGCTCTGTCCAGTGCTGGGGGCACTGA
  GAACCTCATCCTCTTGGAGCAAGAGAAACACCGGCCGCACGAGGTGGGCTCTGTGCAGTGGGCCGGCAGC
  ACCTTCGGGCCCATGCAGAAGAGCCGGCTGGGAGAGCATGAATTTGAAGCCCTCATGAGGATGCTGGACA
  ATTTAGGCTACAGAACAGGCTATACGTACCGCCACCCCTTTGTCCAAGAGAAAACCAAACACAAAAGTGA
  AGTGGAGATCCCAGCCACAGTCACTGCCTTTGTGTTTGAAGAGGATGGAGTCCCAGTCCCCGCCCTGCGC
  CAGCACGCCCAGAAGCAGCAGAAGGAAAAGACCCGCTGGCTTAACACTCCCAACACCTACCTGCGGGTGA
  ACGTGGCTGACGAGGTGCAGAGGAACATGGGCAGTCCCCGACCAAAGACCACGTGGATGAAGGCTGATGA
  AGTGGAAAAGTCCAGCAGCGGCATGCCCATACGGATTGAAAACCCAAACCAATTTGTGCCTCTCTACACT
  GACCCCCAGGAAGTTCTGGACATGAGGAACAAGATTCGAGAGCAAAACCGACAAGACATAAAGTCAGCCG
  GGCCTCAGTCTCAGCTCTTGGCCAGTGTCATCGCAGAGAAGAGCCGGAGTCCGGTACAGCAGAGACTGCC
  CCCAACCGAAGGGGAAGTGTATCAGACTCCTGGGGCTGGGCAGGGGACCCCTGAGTCCTCAGGCCCGCTC
  ACCCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271860
• Insert Size: 1689 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271860.1, NP_001258789.1
• RefSeq Size: 3497 bp
• RefSeq ORF: 1689 bp
• Locus ID: 11519
• UniProt ID: Q9QYB8
• Cytogenetics: 6 37.55 cM
• Gene Summary: This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (5) lacks several 3' exons and contains an alternate 3' exon, and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Both variants 5 and 6 encode the same isoform (2, also known as beta-2).