Zfp451 (NM_001290700) Mouse Untagged Clone

CAT#: MC228307

Zfp451 (untagged) - Mouse zinc finger protein 451 (Zfp451), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp451
• Synonyms: 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; Kiaa0576-hp; mKIAA1702; Znf451
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228307 representing NM_001290700
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGACCCAGGGCCGGAGATAATAGAATCTGTCCCACCAGCTGGGCCCGAGGCATCAGAGTCTACCA
  CGGATGAAAACGAAGATGACATTCAGTTTGTTAGTGAAGGACCGTTAAGACCCGTTTTGGAATACATTGA
  TCTAGTCAGCAGTGATGATGAAGAGCCTAGTACCTCTCACAGTGATAGAATGCCTGAATCTAAAGTGCCA
  TCCTCTGAGAATCATCGCCCAGAAATGTGCTCTAGCTGCAGTGTTCCTCTTCCCATTGGAGACAGCAGCT
  CCTCCTCTGGGAGTTGTGCCAACAGCCCACAAAGGATAGTTTCTCAACCTTCTTCTGTTGAGAACCCATT
  GGAGAACCAGAAAAATGATCACAATAATTCCGATGTTAGTGTCTCTGAAAAAGAGACACTTAAACCACAA
  AGTAATCAGACTCTGCCTTCAGCATCACTTCTGGGCTCCCAGGAATCTTTGGCTTTTTCTGAGGTCAAAG
  AGGATTTACCTGGCACGATGTCTTCAGCTTCACAGAGTGGACAGGATGCCATCCTATATCTCCAGACACA
  AGTGGCTGAGATGTCCCGAGTCATACGTGATCTGCAGTCCAGGAGTTGTTTTAGATTTCATCATTCTAGG
  CCAAGTGAGAGCTCGTCAGTTCCTTGGGATATCTCCAGCTCTAAGGAGGACAGTTTATCCACAGTTGAAG
  AGGAGTCTGACTGCAAATCTCCGTCAGCTGATGACAAAGGGCAGCCACCTGACCCCATGCAGTCTAGTTT
  TACCGGTCTTTTGAAGAGAATGGAACAAAGGGGTGTTATAAAGAGGGTGACGTTCCAGTCTGAAGCAGAA
  ACCTGTGAGGGCAAGCCTGATTGGATGACCTCTAAAAAACGCTTGGTTCCTCCACTGCATCCACTTTTGA
  GAATTGCCACCACTGAAGTTTTTAAAGACCCTGCTGATTGCCATCCTTCTTCCTTCATGGGACACAGGGT
  TTATCCTGTGGCCAAGGACACATCTCCTTTCCAGCCAAATCCACCAGCTGAGGGACCCATCATAGATGTA
  TTAGAACATAGCAAAAGAGGAAACACAGCATCGCCTTTAGACTCTACCTCAAAAGAAATGGAGGTCATGG
  GTTGCAGGTTCTACCATGCTGCTTCCATTGCAGCCCGGGCTGCTAGCTACATGGCCTATATGACTCAGTA
  TCAACGTAAACTCTGGGAAGACATGGAAGATCTGGTTCATGACCCGGAGTTTGATCGTGGGAAAGCAAGG
  TGTATAATATCTGATGGCATGGATGCAGGACTTTGGCAGCTCTGTACCACTAGGGACATAATGGACTCTG
  TAGTCAGAGTTATGGCCATGGCAGTAGACTATAGAAGACAGGCCTGGCTCCGACTAACATCTCTCACTAA
  GAAAACCCAGGAGAAGATCTTACACTTGCCCTTTGATGGTACATCTCTTTTTGGACAAGATGTGAATGCT
  ATTGTTGCAGAAGACAATAGTATTAAAGAAAATGAATATAAGGATCACAATAAATACTATACTCAGCATC
  GGTACTTTTATAGTCATGATCAGAAAGCACATTATCACAACAGAGGGTACTCCAAAGGGGATTGGTACAA
  ACCCCGGAATCACCCCTATAGATACAGAAAAAAGGGAGACTCTCCAGAACGACATGGGTATAAGAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290700
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290700.1, NP_001277629.1
• RefSeq Size: 2077 bp
• RefSeq ORF: 1680 bp
• Locus ID: 98403
• Cytogenetics: 1 12.81 cM
• Gene Summary: E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins. Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11'. Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9'. Plays a role in regulating the transcription of AR targets (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has a distinct 3' structure and 3' terminus, compared to variant 1. The encoded protein (isoform 3) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.