Phf1 (NM_001302397) Mouse Untagged Clone

CAT#: MC228303

Phf1 (untagged) - Mouse PHD finger protein 1 (Phf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf1
• Synonyms: AW557215; D17Ertd455; D17Ertd455e; mPc; Pcl1; PHF; Phf2; Tctex3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228303 representing NM_001302397
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGCTCCCCCGGCTGAGCCGTTTGGGTGCCCCCTCTCTCTGGGATCCAGCTTCCCCTGCTCCCA
  CCTCAGGCCCCAGACCTCGGCTTTGGGAAGGCCAAGATGTGCTGGCCAGATGGACTGATGGGCTGCTGTA
  CTTGGGTACCATCAAAAAGGTGGACAGTGCTCGAGAGGTGTGTCTGGTCCAGTTTGAGGACGATTCCCAG
  TTTCTGGTTCTATGGAAGGATATCAGCCCAGCTGCCCTCCCTGGGGAGGAGCTGCTCTGTTGTGTGTGTC
  GCTCTGAGACCGTGGTACCTGGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCATGCTTATCACCAGGA
  CTGTCACGTTCCCAGGGCCCCGGCCCCTGGAGAAGGAGAGGGCGCATCCTGGGTATGCCGCCAGTGTGTC
  TTTGCAATCGCCACTAAGAGGGGAGGCGCACTGAAGAAAGGTCCCTATGCCCGGGCCATGCTGGGCATGA
  AGCTCTCCCTGCCATACGGATTAAAAGGGCTGGACTGGGATGCTGGACATTTGAGCAACAGACAGCAGAG
  CTACTGCTACTGTGGAGGCCCTGGAGAGTGGAACCTGAAAATGCTGCAGTGCCGGAGCTGCCTCCAGTGG
  TTCCATGAGGCCTGCACCCAGTGTCTGAGCAAGCCCCTCCTCTACGGAGACAGATTCTATGAATTTGAGT
  GCTGTGTGTGCCGGGGCGGCCCTGAGAAGGTCCGGAGGTTACAGCTTCGCTGGGTGGATGTGGCCCATCT
  TGTCCTCTACCACCTCAGCGTTTGCTGTAAGAAGAAATATTTTGATTTTGACCGAGAGATCCTCCCCTTC
  ACCTCTGAGAATTGGGACAGTCTGCTCCTGGGGGAGCTCTCAGATACTCCCAAGGGAGAGCGCTCTTCCC
  AGCTCCTTTCTGCTCTTAACAGCCACAAGGACCGTTTCATTTCAGGGAGGGAGATTAAAAAGCGAAAATG
  TCTTTTTGGTCTCCATGCTCGGACCCCTCCTCCTGTGGAGCTTCTCACTGGAGATGGAGCCCCCACCAGC
  TTCCCTTCAGGGCAGGGCCCTGGGGGAGGGGTCTCACGTCCCCTGGGGAAACGATGGAGGTCGGAGCCAG
  AACCCTTAAGGAGGAGGCAGAAGGGGAAAGTGGAGGAACTGGGGCCACCCACGGCAGCACACAGTCGGCA
  TGGGTCCCGGGAGCAGAGGGCCCTGCAGGCCTCGGTGTCTCCACCACCCCCCAGCCCTAACCAGAGCTAT
  GAGGGCAGCAGCGGCTACAACTTCCGGCCCACAGACGCCCGCTGTCTGCCCAGCCCTATCCGGATGTTCG
  CCTCCTTCCACCCTTCTGCCAGCACTGCAGGGACCTCTGGGGACAGTGAACCCCCAGATAGGTCACCTCT
  GGGACTTCACATTGGCTTCCCCACAGACACCCCTAAAAGTTCACCCCACTCAGTGACGGCCTCATCTTCC
  TCGGTCCCAGCCCTGACCCCAGGCTTTTCCAGACATTCACCCCCTTCTCCCTTGTGCCGTAGTCTGTCTC
  CGGGGACTGGGGGAGGAGTCCGAGGTGGGGTTAGCTACCTGTCCCGAGGGGACCCTGTGAGGGTCCTTGC
  TCGAAGAGTGCGGCCTGACGGCTCTGTGCAGTACCTAGTTGAGTGGGGAGGGGGGGGCATCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302397
• Insert Size: 1677 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302397.1, NP_001289326.1
• RefSeq Size: 2444 bp
• RefSeq ORF: 1677 bp
• Locus ID: 21652
• Cytogenetics: 17 13.6 cM
• Gene Summary: The protein encoded by this gene belongs to the polycomb-like protein family, which is a component of polycomb repressive complex-2. This complex represses gene expression by catalyzing the trimethylation of histone H3 lysine 27 and is required for the regulation of developmental genes including homeotic genes. The gene is expressed primarily in testis tissue. Small interfering RNA-mediated knockdown in cultured cell lines results in changes in homeotic gene expression coincident with alterations in promoter methylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.