Phactr1 (NM_001302636) Mouse Untagged Clone

CAT#: MC228292

Phactr1 (untagged) - Mouse phosphatase and actin regulator 1 (Phactr1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phactr1
• Synonyms: 9630030F18Rik; Rpel1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228292 representing NM_001302636
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTCCGACTCGCTGGTCCCAGGTACGCACACCCCGCCCATCCGGAGGAGGAGTAAGTTTGCCAACC
  TGGGGAGGATTTTCAAACCCTGGAAATGGAGGAAGAAGAAAAGTGAAAAGTTCAAGCACACGTCAGCAGC
  TCTGGAAAGGAAGATTTCGATGAGGCAGAGCAGAGAGGAGCTGATCAAGAGAGGGGTCTTGAAGGAGATC
  TACGATAAAGATGGAGAACTCTCTATATCAAATGAAGATGACTCCCTGGAGAACGGACAGTCCCTGAGCT
  CCAGCCAACTCTCTTTGCCTGCTCTGTCGGAAATGGAGCCTGTCCCAATGCCCAGGGACCCATGCTCATA
  TGAGGTGCTCCAAGCTTCAGACATTATGGATGGACCAGTGTCTGAAGAGAGTCCCTCTGCCAGTGAGTCT
  GGAGTCCTCCTGTCCCAAGATCCTTCAGCCAAACCAGTTCTGTTCCTGCCCCCCAAAAAATCTGCTGCTT
  TCCCTGGAGACCATGAGGAGACCCCAGTGAAACAGTTGTCCCTTCACAAGCAGCCCCCGGCCCTGCCTCC
  CAAACCCACTGCCCGGATTGCCAACCACTTAACAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCA
  GTGAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCCTGATCTGTATGCCTGTAGGGGGGCCAGAGCTCA
  CTCTGGCATCCTATGCAGCCCAGAAGAGCAGCCAGCAGGCCGTGGCCCAGCACCACCACACCGTCCTACC
  ATCCCAGATGCAGCACCAGCTGCAGTATGGCAGTCACGGCCAGCATCTCCCATCCTCCACCGGCACCTTG
  CCCATGCACCCCTCAGGCTGCAGGATGATCGACGAGCTGAACAAGACACTCGCCATGACGATGCAGAGGC
  TGGAAAGCTCCGAGCAACGAGTCCCCTGTTCCACGTCTTACCACAGTTCTGGTTTGCACTCAAGCGACGG
  CATCACAAAAGCAGGACCCATGGGCCTTCCGGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGAT
  GACAATAAGGAAAATGTGCCTCATGAACCCGACTATGAAGACTCGCCCTGCCTGTATGGCCGGGAGGAGG
  AGGAAGAGGAGGAAGATGAAGATGATGACGCCTCTTTGTACACCAGCTCACTGGCCATGAAGGTGTGCAG
  GAAGGACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCTAAGCGAGAGCTAGAAGAAAAGAACATCCTC
  CCCAGACAGACGGATGAGGAACGGCTGGAGCTCAGACAGCAGATTGGCACCAAGCTCACCAGGCGGCTGA
  GCCAGAGACCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAGCCTCGGAATGAACAAGAAGAACA
  GGAGGAGAAGCGGGAAATCAAGAGGAGGCTGACTCGCAAGCTCAGCCAAAGGCCCACAGTGGAAGAACTC
  CGGGAGAGGAAGATCCTTATCCGCTTCAGTGACTACGTGGAAGTGGCAGATGCCCAAGACTATGACCGAA
  GGGCAGACAAGCCATGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATTCGGAAGGAACTCAATGAGTT
  TAAAAGTACCGAGATGGAAGTCCACGAGTTAAGTAGACACTTAACCAGGTTTCATCGACCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302636
• Insert Size: 1674 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302636.1, NP_001289565.1
• RefSeq Size: 5321 bp
• RefSeq ORF: 1674 bp
• Locus ID: 218194
• UniProt ID: Q2M3X8
• Cytogenetics: 13 A4
• Gene Summary: Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization (PubMed:30256902).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks exons in the 5' coding region, and uses a downstream start codon compared to variant 2. The resulting isoform (5) has a shorter N-terminus compared to isoform 2.