Tiam2 (NM_001286758) Mouse Untagged Clone

CAT#: MC228288

Tiam2 (untagged) - Mouse T cell lymphoma invasion and metastasis 2 (Tiam2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tiam2
• Synonyms: 3000002F19Rik; mKIAA2016; STEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228288 representing NM_001286758
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCACTTTTTGGGAGCCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGG
  ATGCGATCTCCGCTTCCTCGGACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTC
  CCTTGGAGGTTCTTTCCTCTACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATT
  AAAGTACAGAGGGTTCTAGAGCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATC
  CCACCAAGCAGCACTCCTCCACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCC
  TCTGCTTCTCAAGGAGCTAGTGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCA
  CTAAAGGCCATGGAAAAAGTCGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGG
  TGTTTGACCAGCTGGTGGCAGAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACT
  TCTGATGCACTCTACAGTTTCCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAG
  CTCACAGTATTTGTTTTTAAGAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAAC
  TGCCCTCGAATTCCCGGCCTGCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCC
  CATATCTGCGCTTCAAGTTAGACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATT
  CATACCAAGTCGGAAATTGAAGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACA
  AAACCAGCATTGTTAAGGTGATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCT
  GCCACTGGAGAAGACGTGTAAGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTA
  GCCTCGTCCAGGTCGTTGAAGGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGG
  GCAACTCACTGGACTCAGATGAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGA
  GAGCAGGCGAGACTCTAAGAGCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGAT
  GGTCTTATCAAAGAAAGCGACATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTA
  GCCCTACTAAGGACATTGAGATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGT
  TGGGCAGCAGCCTCTCACAGAGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAA
  CGGAAAGCAAACAGCACCAAGAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGG
  ACAGCCACCCAGAAACTGCCAGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTT
  AACTTCAGTCGTCAATGAGGAGGGTTTTTATGAAACACAGAGCCATGGCAAATCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286758
• Insert Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286758.1, NP_001273687.1
• RefSeq Size: 2550 bp
• RefSeq ORF: 1668 bp
• Locus ID: 24001
• UniProt ID: Q6ZPF3
• Cytogenetics: 17 1.99 cM
• Gene Summary: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.