Cstf2 (NM_001290398) Mouse Untagged Clone

CAT#: MC228278

Cstf2 (untagged) - Mouse cleavage stimulation factor, 3' pre-RNA subunit 2 (Cstf2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cstf2
• Synonyms: C630034J23Rik; Cstf64; mir-3112
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228278 representing NM_001290398
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTTTGCCAGTGAGAGACCCTGCGGTGGATCGTTCCCTGCGCTCCGTCTTCGTGGGAAACATCC
  CTTATGAAGCTACTGAAGAACAACTGAAGGACATTTTCTCTGAGGTTGGGCCTGTTGTTAGTTTCAGATT
  GGTATACGATAGGGAGACAGGAAAACCAAAGGGCTATGGCTTCTGCGAGTACCAAGACCAAGAGACAGCA
  CTTAGTGCCATGCGGAACTTGAATGGGCGTGAATTCAGCGGGAGAGCACTTCGAGTGGATAATGCTGCCA
  GTGAAAAGAACAAGGAGGAACTGAAGAGCCTTGGCACTGGTGCACCTGTCATAGAGTCACCTTATGGAGA
  AAGCATCAGCCCTGAGGATGCCCCTGAATCCATTAGCAAAGCAGTTGCCAGTCTTCCACCAGAACAGATG
  TTTGAGTTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAAGCACGAAACATGTTGCTTC
  AGAATCCACAGCTGGCTTATGCTTTGTTGCAAGCACAGGTAGTGATGAGAATTGTGGATCCTGAGATTGC
  GCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTGATTTCAGGCAACCCTCAGCCAGTTCATGTT
  GCTGGGCCTGGCTCAGGACCCAATGTTTCAATGAACCAGCAGAATCCGCAGGCCCCTCAGGCTCAATCTT
  TGGGTGGAATGCATGTCAATGGCGCACCTCCTATGATGCAAGCTTCTATGCCAGGTGGAGTTCCAGCCCC
  AGTGCAGATGGCAGCTGCTGTAGGAGGACCTGGCCCTGGTTCCTTAGCCCCTGCAGGAGTTATGCAAGCC
  CAAGTTGGAATGCAAGGAGCTGGACCAGTGCCCATGGAACGAGGACAAGGTGATGCTCCCAATGACCCGC
  GGGGAGGCACTTTAATGACTGTAACTGGAGACGTAGAACCTAGAGCTTACCTGGGACCACCACCACCACC
  TCATCAGGGCCCACCCATGCACCATGTTCCTGGTCATGAAGGTCGTGGACCGCCTCCACATGACATGAGA
  GGAGGACCCCTAGCTGAGCCGAGACCTTTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGCC
  CACCTCTGGATGCCAGAGGTGGAAGAGATCCCCGAGGATTAGATGCACGGGGGATGGAGGCCAGAGCCAT
  GGAAGCAAGAGGACTAGATGCCAGAGGACTGGAAGCCCGTGCCATGGAGGCCCGTGCCATGGAGGCCCGT
  GCCATGGAAGCCCGTGCCATGGAAGCCCGTGCCATGGAGGCTCGTGCCATGGAGGCCAGAGGCATGGACA
  CCAGAGGCCCAGTACCTGGACCTAGAGGACCTATGCCTAGTGGAATCCAAGGTCCCAATCCAATGAACAT
  GGGGGCAGTTGTTCCCCAGGGATCCAGACAGGTACCAGTCATGCAGGGAGCAGGCATGCAAGGAGCAAGC
  ATGCAAGGTGGAAGCCAGCCTGGTGGCTTTAGTCCTGGACAGAGCCAAGTCACACCCCAGGATCATGAGA
  AGGCTGCTTTGATTATGCAGGTCCTTCAGCTCACTGCAGACCAGATCGCCATGCTGCCTCCTGAGCAAAG
  ACAGAGTATCCTAATCTTAAAGGAACAGATACAGAAATCCACTGGGGCACCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290398
• Insert Size: 1665 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290398.1, NP_001277327.1
• RefSeq Size: 4464 bp
• RefSeq ORF: 1665 bp
• Locus ID: 108062
• Cytogenetics: X E3
• Gene Summary: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).[UniProtKB/Swiss-Prot Function]