Me1 (NM_001198933) Mouse Untagged Clone

CAT#: MC228270

Me1 (untagged) - Mouse malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Me1
• Synonyms: D9Ertd267e; Mdh-1; Mod-1; Mod1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228270 representing NM_001198933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGATCTGCGAAGACTTGGCTTTTACTCTGGAAGAGAGACAGCAGTTGAACATTCATGGATTGT
  TGCCGCCCTGCATCATCAGCCAGGAGCTCCAGGTCCTTAGAATAATTAAGAATTTCGAACGACTGAACTC
  TGACTTCGACAGGTATCTCCTGTTAATGGACCTGCAAGACAGAAATGAGAAGCTCTTCTACAGCGTGCTC
  ATGTCTGATGTTGAAAAGTTCATGCCTATTGTTTACACCCCCACCGTGGGCCTCGCATGCCAGCAGTACA
  GTTTGGCATTCCGGAAGCCAAGAGGCCTCTTTATTAGTATCCATGACAAAGGGCACATTGCTTCAGTTCT
  TAATGCATGGCCAGAGGATGTCGTCAAGGCTATTGTGGTAACTGATGGAGAGCGCATCCTTGGCTTGGGA
  GACCTTGGCTGTAATGGGATGGGCATCCCTGTGGGTAAACTGGCCCTGTACACGGCATGTGGAGGGGTGA
  ACCCACAACAGTGTCTACCCATCACTTTGGATGTGGGAACAGAAAATGAGGAGTTACTTAAGGATCCACT
  GTACATCGGGCTGCGGCACCGGCGAGTCAGAGGCCCTGAGTATGACGCCTTCCTGGATGAGTTCATGGAG
  GCAGCGTCTTCCAAATATGGCATGAATTGCCTTATTCAGTTTGAAGATTTTGCCAATCGGAATGCATTTC
  GTCTCCTGAACAAGTATCGAAACAAGTATTGCACATTTAACGATGATATTCAAGGAACAGCGTCTGTTGC
  GGTTGCGGGTCTCCTTGCAGCTCTTCGAATAACCAAGAACAAGCTCTCTGATCAGACAGTGCTGTTCCAG
  GGAGCTGGAGAGGCTGCCTTGGGGATTGCTCACTTGGTTGTTATGGCCATGGAGAAAGAAGGTTTATCAA
  AGGAGAATGCTAGAAAGAAGATATGGTTGGTTGACTCAAAAGGACTAATAGTTAAGGGTCGTGCATCTCT
  CACAGAAGAGAAAGAGGTGTTTGCCCATGAACATGAAGAAATGAAGAATCTGGAAGCCATTGTTCAAAAG
  ATAAAACCAACTGCCCTCATAGGAGTTGCTGCAATTGGTGGTGCTTTCACTGAACAAATTCTCAAGGATA
  TGGCTGCCTTCAACGAGCGGCCCATCATCTTTGCTTTGAGTAATCCGACCAGCAAAGCGGAGTGCTCTGC
  AGAGCAGTGCTACAAGGTGACCAAGGGACGTGCAATCTTTGCCAGCGGCAGTCCTTTTGATCCAGTCACT
  CTCCCAGATGGACGGACTCTGTTTCCTGGCCAAGGCAACAATTCCTACGTGTTCCCTGGAGTTGCTCTTG
  GGGTGGTGGCCTGCGGACTGAGACACATCGATGATAAGGTCTTCCTCACCACTGCTGAGGTCATATCTCA
  GCAAGTGTCAGATAAACACCTGCAAGAAGGCCGGCTCTATCCTCCTTTGAATACCATTCGAGGCGTTTCG
  TTGAAAATTGCAGTAAAGATTGTGCAAGATGCATACAAAGAAAAGATGGCCACTGTTTATCCTGAACCCC
  AAAACAAAGAAGAATTTGTCTCCTCCCAGATGTACAGCACTAATTATGACCAGATCCTACCTGATTGTTA
  TCCGTGGCCTGCAGAAGTCCAGAAAATACAGACCAAAGTCAACCAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198933
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198933.1, NP_001185862.1
• RefSeq Size: 3335 bp
• RefSeq ORF: 1659 bp
• Locus ID: 17436
• UniProt ID: P06801
• Cytogenetics: 9 46.58 cM