Coch (NM_001198835) Mouse Untagged Clone

CAT#: MC228268

Coch (untagged) - Mouse coagulation factor C homolog (Limulus polyphemus) (Coch), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Coch
• Synonyms: AW122937; Coch-5B2; D12H14S564E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228268 representing NM_001198835
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGTCCAGGATCCCTGCTCTCTGCCTCGGTGCGTGGCTGCTGCTGCTGCTGCTGCCCCGGTTCG
  CGCGCGCCGAGGGAGCGGTTCCCATTCCTGTCACCTGCTTTACCAGAGGCCTGGATATCCGAAAAGAGAA
  AGCAGATGTTCTCTGCCCAGGAGGCTGCTCTCTTGAGGAATTCTCTGTGTTTGGGAACATAGTGTATGCG
  TCAGTGTCCAGCATCTGCGGCGCTGCTGTCCATAGGGGAGTGATTGGCACCTCAGGGGGACCTGTGCGTG
  TCTACAGCCTTCCTGGTCGAGAGAACTACTCCTCGGTAGATGCCAACGGCATCCAGTCTCAGATGCTTTC
  CCGATGGTCCGCGTCCTTCGCTGTGACCAAAGGCAAAAGCAGTACCCAGGAAGCCACAGGACGGGCAGTG
  TCCACAGCCCACCCACCTTCAGGTAAAAGACTAAAGAAGACACCAGAGAAGAAGACTGGCAACAAAGACT
  GTAAGGCAGACATTGCATTTCTCATTGATGGAAGCTTCAATATTGGGCAGCGCCGATTTAATTTGCAGAA
  GAATTTTGTTGGGAAAGTGGCACTAATGTTGGGAATTGGAACAGAAGGACCACACGTGGGTCTCGTTCAA
  GCCAGTGAACACCCCAAAATAGAATTTTACTTGAAAAACTTTACTTCAGCCAAAGATGTCTTGTTTGCCA
  TAAAAGAAGTAGGTTTCCGAGGGGGTAACTCCAACACAGGAAAAGCCTTGAAGCACACTGCTCAGAAATT
  CTTTACAGCAGACACTGGTGTGAGAAAAGGAATACCAAAAGTGGTGGTAGTGTTTATTGATGGTTGGCCC
  TCTGATGACATTGAGGAAGCAGGCATTGTGGCCAGAGAGTTTGGTGTCAATGTATTTATAGTTTCTGTGG
  CCAAGCCCATTCCTGAAGAACTGGGGATGGTTCAAGATGTTGCATTTGTTGACAAGGCTGTGTGTCGGAA
  TAATGGCTTCTTCTCTTATCACATGCCCAACTGGTTTGGCACTACAAAATATGTGAAGCCTCTGGTGCAG
  AAGCTCTGTACGCACGAACAGATGATGTGCAGCAAAACCTGCTACAACTCAGTGAACATTGCCTTTCTGA
  TTGACGGCTCCAGCAGTGTTGGAGATAGCAATTTCCGCCTCATGCTAGAATTTGTTTCTAACATAGCGAA
  GACATTTGAAATCTCAGACATTGGAGCCAAGATAGCTGCTGTACAGTTCACTTATGACCAGCGCACCGAG
  TTCAGTTTCACTGACTATAATACCAAAGAGAACGTCCTAGCTGTCCTAGCGAACATCCGCTACATGAGTG
  GTGGCACAGCTACTGGTGATGCCATCGCCTTTACTGTTAGAAATGTATTTGGTCCCATAAGGGACAGCCC
  CAACAAAAACTTCCTGGTTATTGTCACAGATGGGCAGTCCTATGATGATGTCCGAGGCCCTGCTGCAGCT
  GCCCATGATGCAGGTATCACCATCTTCTCTGTTGGTGTGGCTTGGGCACCGCTGGATGACCTGAGAGATA
  TGGCCTCTAAACCCAAAGAGTCACACGCTTTCTTTACCAGAGAGTTCACAGGGTTAGAACCAATTGTCTC
  TGACGTCATCAGAGGCATTTGTAGAGACTTCTTAGAATCCCAGCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198835
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198835.1, NP_001185764.1
• RefSeq Size: 2484 bp
• RefSeq ORF: 1659 bp
• Locus ID: 12810
• UniProt ID: Q62507
• Cytogenetics: 12 22.11 cM
• Gene Summary: Plays a role in the control of cell shape and motility in the trabecular meshwork.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.