Cnot6l (NM_001285511) Mouse Untagged Clone

CAT#: MC228262

Cnot6l (untagged) - Mouse CCR4-NOT transcription complex, subunit 6-like (Cnot6l), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cnot6l
• Synonyms: 4932442K20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228262 representing NM_001285511
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAAGGAAAAATATGATCCTCCAGATCCTCGCAGAATTTACACCATCATGTCCGCAGAGGAGGTAG
  CCAATGGGAAAAAATCTCACTGGGCAGAGTTAGAGATCTCGGGTAGAGTGCGGAGCTTAAGTACATCACT
  CTGGTCATTAACACACTTGACAGCACTGCACCTAAATGACAATAACCTTGCTCGCATTCCACCTGATATT
  GCCAAGCTTCATAATCTGGTTTACCTGGATCTGTCATCCAATAAACTCAGAAGTTTACCAGCAGAACTAG
  GAAACATGGTGTCTCTCAGGGAATTGCTTTTAAATGACAATTATTTACGGGTGTTGCCTTATGAACTTGG
  CCGGCTCTTCCAGCTACAAACTCTAGGTTTAACAGGCAATCCTTTATCACAGGATATTATGAGCTTATAC
  CAGGACCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAATCTTGCAGTTCATCCAGAGCAGC
  TTCCTCCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTGCCATCAGCATCATTCACGGTTAT
  GTGTTACAATGTGTTATGTGATAAATATGCTACCAGGCAGCTATATGGCTATTGTCCGTCCTGGGCATTA
  AACTGGGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAACTGGGACGCAGATATCATTAGTCTTC
  AGGAAGTGGAAACAGAGCAATACTTTACTCTCTTTCTGCCAGCATTGAAGGATCGTGGATATGATGGATT
  TTTTTCTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAAAGAAAGCATGTGGATGGTTGTGCAATA
  TTCTTCAAAACAGAAAAATTTACATTGGTGCAGAAGCATACAGTGGAATTCAACCAGGTAGCAATGGCAA
  ATTCAGATGGATCCGAAGCAATGCTAAACAGAGTAATGACGAAAGATAACATTGGCGTTGCTGTGGTGTT
  AGAGGTCCACAAGGAGCTTTTTGGAACAGGTATGAAGCCTATTCATGCTGCAGACAAACAGCTGCTTATA
  GTGGCAAATGCCCACATGCATTGGGACCCAGAGTATTCTGATGTGAAACTTATTCAGACCATGATGTTTG
  TCTCAGAGGTTAAAAACATTCTGGAGAAAGCCTCAAGTAGGCCTGGCAGCCCAACTGCAGATCCCAATTC
  CATCCCGCTGGTGCTATGTGCAGATCTTAACTCATTGCCAGATTCAGGTGTTGTGGAATATTTAAGCAAC
  GGTGGAGTAGCTGACAACCATAAAGACTTCAAGGAACTAAGGTACAATGAGTGTCTTATGAACTTCAGCT
  GTAGTGGAAAGAATGGAAGCTCAGAAGGGAGAATCACACATGGCTTCCAACTTAAGAGCGCCTATGAAAA
  TAACTTGATGCCTTATACCAATTACACCTTTGATTTCAAAGGTGTGATTGACTACATTTTCTATTCCAAG
  ACTCATATGAACGTGCTTGGTGTCCTGGGGCCTTTAGATCCTCAATGGCTGGTTGAGAACAACATCACTG
  GGTGTCCACACCCTCACATCCCTTCAGACCACTTCTCACTGTTAACACAACTTGAACTCCACCCTCCACT
  CCTGCCTCTTGTCAATGGTGTTCACTTGCCTAATCGGAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285511
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285511.1, NP_001272440.1
• RefSeq Size: 8696 bp
• RefSeq ORF: 1653 bp
• Locus ID: 231464
• UniProt ID: Q8VEG6
• Cytogenetics: 5 E3
• Gene Summary: Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 2. Variants 1 and 3 encode the same isoform (1), which is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.