Ptprr (NM_001161837) Mouse Untagged Clone

CAT#: MC228260

Ptprr (untagged) - Mouse protein tyrosine phosphatase, receptor type, R (Ptprr), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprr
• Synonyms: Gmcp1; mPTP213; PTP-SL; PTPBR7; RPTPRR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228260 representing NM_001161837
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCGCAACACTCGGTCAGTTTCCACACCGACACTGCAAATGGATATAACCAAGCTGAACATAACCC
  TGCTTCGGATCTTCCGCCAAGGAGTAGCTGCAGCCCTGGGACTCTTACCTCAGCAAGTGCACATTAACCG
  GCTCATTGAAAAGAAGAACCAGGTTGAGTTGTTTGTGTCTCCCGGAAACCGGAAACCAGGAGAAACGCAG
  GCCCTGCAGGCTGAGGAAGTGCTGCGTTCCCTCAATGTGGATGGTTTGCATCAGAGTTTACCACAGTTTG
  GAATTACAGACGTCGCCCCTGAGAAAAATGTTTTACAAGGGCAGCACGAAGCAGACAAGATCTGGAGCAA
  AGAAGGATTTTACGCTGTCGTCATCTTCCTCAGCATCTTTATCATCATAGTAACCTGTTTGATGATTATT
  TACAGGTTAAAAGAAAGGCTTCAGCTTTCCTTAAGACAAGATAAAGAGAAAAACCAGGAGATCCACCTAT
  CACCCATTGCACGGCAGCAAGCACAATCGGAGGCCAAGACGACCCACAGCATGGTCCAGCCCGATCAGGC
  GCCAAAGGTGCTGAACGTGGTTGTGGACCCTCAAGGCCAATGCACTCCTGAGATTCGAAACAGCACCTCC
  ACCTCTGTCTGCCCTTCTCCCTTCAGAATGAAGCCCATAGGACTCCAGGAGCGACGAGGTTCCAATGTAT
  CTCTTACGCTGGACATGAGTAGCCTGGGCAGTGTGGAACCCTTTGTGGCCGTCTCAACCCCCCGGGAGAA
  GGTAGCCATGGAATACCTGCAGTCAGCCAGCCGAGTTCTCACACGGTCACAGCTGAGGGACGTCGTGGCA
  AGTTCCCACCTACTTCAAAGTGAATTCATGGAAATACCAATGAATTTTGTGGATCCCAAAGAAATTGATA
  TTCCACGTCACGGAACTAAAAATCGTTATAAGACCATTTTGCCAAATCCCCTCAGCAGAGTGTGCTTAAG
  ACCAAAAAATATAACCGATTCCTTGAGTACTTACATAAATGCTAACTATATTCGGGGCTACAGTGGTAAG
  GAGAAAGCCTTCATTGCCACCCAGGGCCCCATGATCAACACTGTGAATGACTTCTGGCAGATGGTGTGGC
  AAGAAGACAGTCCCGTGATTGTGATGATCACGAAACTCAAAGAGAAAAATGAGAAATGTGTGCTCTACTG
  GCCAGAAAAGAGAGGGATTTACGGCAAGGTTGAGGTTCTGGTCACCGGTGTGACCGAATGTGATAACTAC
  ACCATCCGCAACCTCGTCTTAAAGCAAGGAAGTCACACCCAACATGTGAAGCACTACTGGTACACTTCAT
  GGCCGGATCATAAGACTCCAGACAGTGCCCAGCCCCTTCTGCAGCTCATGTTGGATGTGGAAGAAGACAG
  ACTGGCCTCTGAAGGCCGAGGGCCTGTGGTTGTCCACTGCAGTGCGGGGATTGGGAGAACTGGGTGTTTC
  ATCGCTACATCCATTGGCTGTCAACAATTGAAAGAAGAAGGAGTTGTAGACGCACTAAGTATTGTCTGCC
  AGCTTCGTGTAGACAGGGGTGGTATGGTCCAAACCAGCGAGCAGTATGAATTTGTGCACCATGCTCTGTG
  CCTGTTCGAGAGCAGACTTTCACCAGAAACTGTCGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161837
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161837.1, NP_001155309.1
• RefSeq Size: 2860 bp
• RefSeq ORF: 1650 bp
• Locus ID: 19279
• UniProt ID: Q62132
• Cytogenetics: 10 D2
• Gene Summary: Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.