Ddhd1 (NM_001284399) Mouse Untagged Clone

CAT#: MC228251

Ddhd1 (untagged) - Mouse DDHD domain containing 1 (Ddhd1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddhd1
• Synonyms: Mir5131; PA-PLA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228251 representing NM_001284399
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAGTCTATCTCTACAGTGACGCAACAACATCCAAAATTGCGAGAACCGTTACCCAAAAACTGG
  GCTTTTCTAAAGCCTCCAGCAGTGGGACCAGACTGCACAGAGGTTATGTAGAAGAAGCCACACTGGAAGA
  CAAGCCGTCGCAGACTTCTCATATCGTGTTTGTTGTGCACGGCATTGGACAGAAGATGGACCAAGGAAGA
  ATCATCAAAAACACAGCCATGATGAGAGAGGCTGCAAGGAAAATGGAAGAAAAGCATTTTTCCAACCATG
  CAACACATGTTGAATTTCTGCCTGTTGAATGGCGATCAAAGCTTACTCTTGATGGAGACACAGTTGATTC
  CATCACTCCTGACAAAGTGCGAGGCCTGAGGGACATGCTCAACAGCAGCGCGATGGACATCATGTACTAC
  ACCAGCCCACTCTACAGGGATGAGCTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTATATTCCCTTT
  TCTGTTCCCGGAATCCAGACTTTGAAGAGAAAGGGGGTAAAGTCTCAATAGTGTCCCATTCCTTGGGGTG
  TGTAATCACTTATGACATCATGATGGGCTGGAATCCAGGTGGACTCTATGAGCAGCTGCTGCAGAAGGAG
  GAAGAGCTGCCCGACGAGCGGTGGATGAGCTATGAAGAGCGGCATCTTCTTGACGAGCTCTATATAACAA
  AGCGACGGCTGAGGGAAATAGAAGATCGGCTGCACGGATTGAAGGCGCCATCCATATCACAAACACCTGC
  CTTAAAATTTAAGGTTGAAAATTTCTTCTGTATGGGATCTCCGCTAGCAGTTTTTTTGGCATTACGCGGC
  ATCCGACCAGGAAATTCTGGAAGTCAAGACCACATTTTACCTAGAGAGATCTGTAACCGCTTACTAAATA
  TTTTTCATCCTACGGACCCAGTGGCCTACAGATTAGAACCGTTGATTTTGAAACACTACAGCAACATTTC
  TCCAGTCCAGATCCACTGGTACAACACTTCAAACCCTCTACCTTATGAGCATATGAAGCCAAATTTTCTC
  AACCCAGCAAAAGAACCTACCTCAGTTTCAGACAGTGAGAACATAGCAGCTATCCCAAGCCCTGTGACCT
  CCCCCGTCTTATCTCGGCGCCACTATGGAGAATCTATAACTAACATTGGCAAAGCAAGCATATTGGGGGC
  TGCTAGCATTGGAAAGGGCCTTGGAGGAATGTTGTTCTCAAGATTTGGACGTTCTTCAGCATCACAGCCG
  TCTGAGCCATCTAAAGACTCACTGGAAGATGATAAGAAGCCTTCAGCTTCACCCTCTACCACCACCGTGG
  CAACGCAGACCCTGCCGCACAGTGGCTCCGGCTTCCTCGACTCCGCATATTTCAGACTTCAAGAATCGTT
  CTTTTATCTCCCACAACTTCTTTTTCCGGAAAATGTAATGCAGAGTAAAGATGATAGCCTCGTGGAACTG
  GAACACAGGATCGACTTTGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTCAGCCGTCACGTCGC
  ACACTGCCTATTGGTCATCCTTGGATGTCGCTCTTTTTCTTTTAACATTCATGTACAAACACGAGCATGA
  TACCGAGGCCAAGCCCAGCCTAGGTTCACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284399
• Insert Size: 1644 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284399.1, NP_001271328.1
• RefSeq Size: 5173 bp
• RefSeq ORF: 1644 bp
• Locus ID: 114874
• UniProt ID: Q80YA3
• Cytogenetics: 14 C1
• Gene Summary: Phospholipase that hydrolyzes phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid. The different isoforms may change the substrate specificity (By similarity). Required for the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 3. The encoded isoform (4) has a shorter N-terminus, compared to isoform 3.