Trim26 (NM_001286727) Mouse Untagged Clone

CAT#: MC228246

Trim26 (untagged) - Mouse tripartite motif-containing 26 (Trim26), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim26
• Synonyms: AI462198; Zfp173; Zfp1736
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228246 representing NM_001286727
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGTCAGCCCCCTTGAGGAGCCTGGAGGAAGAGGTGACCTGCTCCATCTGCCTGGATTACCTGA
  GGGACCCGGTGACCATCGACTGTGGCCATGTCTTCTGTCGCAGCTGCACAAGTGATATCCGCCCCATATC
  GGGGAACCGGCCTGTCTGCCCGCTCTGCAAGAAGCCCTTTAAGAAGGAGAACATCCGACCCGTGTGGCAG
  CTAGCAAGTCTGGTGGAGAACATTGAACGGCTGAAGGTGGACAATGGCAGACAGCCAGGAGAGCTCGCCC
  GCGAGCCACAGGACATGAAGTTGTGTGAGCGGCACCAGGAGAAGCTACACTACTACTGTGAAGATGATGG
  CAAACTGTTGTGTGTGATGTGCAGGGAGTCCCGGGAGCACCGCCCCCACACTGCAGTCCTGGTGGAGAAA
  GCCGCCCTGCCTCACAGAGAGAAAATTCTGAACCACTTGAACACCCTAAGGAGAGACAGAGACAAAATCC
  AGGGCTTTCAAGCCAAGGGAGAAGCTGATATTCTGGCTGCACTGACAAAGCTGCAGGAGCAGAGGCAGTA
  CATTGTGGCGGAATTTAAGCAGGGCCACCAGTTTCTAAAGAAGCGGGAGCAGCACCTGCTAGACCAGCTG
  GCCACCTTGGAGCAGCTCCTCACCGAGGGCAGGGAGAAGTTCAAGACCCGGGGCGTCAGTGAGCTTGACC
  GGTTGACTCTGGTCATCTCCGAGCTGGAGGGCAAGGCACGACAGCCAGCTGCAGAGCTGATGCAGGATGT
  CTGCACTACACAGGACACCAAGGACTTCGCCAACAAGTACCCACGGAAGAAGTTCTGGATTGGGAAAGCC
  ATCCCTCACATGGTTAAAAGAAAGGCAGGAGAATTCTCAGATAAACTTCTTTCTCTGCAGCGAGGCCTGA
  GGCAGTTCCAGGGCAAGCTGCTGAGAGACTTGGAGTATAAGACAGTAAGCGTCACCCTGGACCCACAGTC
  GGCCAGTGGATACCTACATCTTTCAGAGGACTGGAAGTGTGTGACCTATACTGGCCAATACCAGAGTGAC
  TGTCTGCTCCCCCAGCAGTTTGACTGTGAGCCAGGAGTGTTGGGCAGCAAGGGCTTCACATGGGGAAAGG
  TATACTGGGAAGTAGAGTTGGAGAGAGAAGGCTGGTCAGAGGATGAAGAAGAGGGGGAGGAGGAAGAAGA
  AGGGGAAGAAGAGGAAGAAGACGAGGAGGTTGGCTATGGGGACGGATACGAAGACTGGGAAACAGATGAA
  GAAGACGAATCACTGGGGGAAGAGGAAGAGGAGGAAGAGGAAGAAGAAGAAGAAGTTCAGGAAAGCTGCA
  TGGTGGGAGTGGCCAAAGACTCTGTGAAGAGGAAAGGGGACCTCTCCCTGCGACCAGAAGATGGTGTGTG
  GGCCCTTCGGCTCTCCCCCTCAGGTATCTGGGCCAACACGAGCCCAGAGGCCCAGCTGTTCCCGGTGCTG
  CGGCCCCGGAGAGTGGGCATCGCCCTGGATTATGAAGGTGGCACTGTGACATTCACCAATGCAGAGTCCC
  AGGAACTCATCTATACCTTCACGACCACCTTCACCCGGCGCCTGGTTCCCTTCCTGTGGCTCAAGTGGCC
  AGGAGCACGCCTTCTGCTGAGACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286727
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286727.1, NP_001273656.1
• RefSeq Size: 3199 bp
• RefSeq ORF: 1638 bp
• Locus ID: 22670
• UniProt ID: Q99PN3
• Cytogenetics: 17 19.16 cM
• Gene Summary: E3 ubiquitin-protein ligase which regulates the IFN-beta production and antiviral response downstream of various DNA-encoded pattern-recognition receptors (PRRs). Promotes nuclear IRF3 ubiquitination and proteasomal degradation. Bridges together TBK1 and NEMO during the innate response to viral infection leading to the activation of TBK1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has an alternate splice junction in the 5' UTR, compared to variant 3. Variants 1, 3 and 4 encode the same protein.