Ippk (NM_001276399) Mouse Untagged Clone

CAT#: MC228221

Ippk (untagged) - Mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ippk
• Synonyms: 1810043M15Rik; InsP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228221 representing NM_001276399
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGTGCGTGCCGCGTGGCCTCACACCGTGCAGCCTCCCCCGGGATGCTCGTCTTACTGTCAGGCA
  ACGGTGGGCCAGAGGCTGAGCCTGGACCAGGGCCTGGGCAGCACAGGGGAAAGTTCCCAGAGTGCTGTCA
  GCCCACCGAGTGGAGTTCAGCCTCGAGAAACCACAGAGCTGGTGTTCTGCCTTGCTGCTGCGCTGTCAGG
  AACGGGGCGCTGCCACAGCGCTGTGTAGTTCTGCGTTTTCTGAAGTTTCCTCCAAATAAGAAGAAGACCT
  CAGAAGAGATACTTCAGCATCTGCAGAACATAGTAGACTTTGGCAAAAATGTCATGAAAGACTTTTTGGG
  AGAGAACTATGTTCATTGTGGGGAAGTTGTTCAGCTGCCTTTAGAGTTTGTGAAACAACTCTGTTTAAAG
  ATACAGTGTGAAAGACCAGAGTCCCGTTGTGACAAGGACCTGGACACATTCAGTGGTTATGCTATGTGCC
  TTCCCAATTTAACCAGACTTCAGACCTTCCACTTTGCAGAGCACCGGCCGATTCTGTGTGTAGAGATTAA
  GCCAAAATGTGGGTTTATTCCTTTCTCAAATGATGTTACCCACGAGATGAAGCACAAGGTGTGCCGATAC
  TGCATGCACCAGCACCTCAAGGTAGCAACTGGAAAGTGGAAGAAAATCAGTAAATACTGTCCCCTCGACC
  TCTACTCAGGAAATAAGCAGCGCATGCACTTTGCCCTCAGAAGTCTGCTGCAGGAGACACAGAATAATCT
  TAGGATATTTAAGAATGGAGAGCTGATTTATGGTTGTGGTGATGCCCGGAGCCCTGTGGCTGACTTGAAG
  GAGCTTGCACACCACCTGAAGCCCTTCTTCTTCCCTTCCAACGGCTTGGCCAGTGGGCCTCACTGCACGA
  AGGCTGTGATCCGTGAGCTGGTGCATGTTATCACCAGGGTGCTGCTGAGCAGCTCAGAAAAAGCCCGAGC
  TGGTGCCCTGAGGCTTGGGCTCCAGGGCCCACGAGTCTGTGAAGCCAGTCCTTTCAGCAGGAGCCTACAC
  AACCAAGGAAAAAACACCTCAGAGCACTCGGGGTTACCGAAGGGCTGTCTTCTGTACAAAACCCTCCAGG
  TGCAGATGTTGGACCAGCTGGACATCGAAGGCCTCTACCCTCTGTACAAACGGGTTGAGCAATACCTGGA
  GGAGTTTCCTGAGGAGAGGAAAACATTACAGATAGATGGGCCTTATGATGAAGTATTTTACCAGAAACTG
  CTTGATCTGTCCACTGAGGATGATGGGACAGTGGCCTTTGCATTAACAAAGGTGCAGCAATACAGAGTAG
  CCATGACTGCCAAGGACTGTTCCATCATGATCGCACTTTCCCCTTGTCTACAGGGCACCAGCTCTGACCA
  GAGGCCTGTTATTCCTTCGTCAAGATCCCGGCTGGCCTTCTCTGTGTCTGTATTGGACCTCGACCTCAAG
  CCCTATGAGAGCATTCCTCATCAGTATAAACTAGACAGCAAGATAGTCAACTATTACTCAAAGACTGTGC
  ATGCCAAAGATGACACTGTGAGGTCGACTCGGTTCAAGGAACATGAAGATTGCACATTAGTTCTCCATAA
  GGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276399
• Insert Size: 1617 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276399.1, NP_001263328.1
• RefSeq Size: 3015 bp
• RefSeq ORF: 1617 bp
• Locus ID: 75678
• Cytogenetics: 13 A5
• Gene Summary: Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 5' exon and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is longer and has a distinct N-terminus, compared to isoform a.