Tti2 (NM_001199988) Mouse Untagged Clone

CAT#: MC228217

Tti2 (untagged) - Mouse TELO2 interacting protein 2 (Tti2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tti2
• Synonyms: MGC28346
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228217 representing NM_001199988
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGGACAGTGCCGAAGAGAAGTCGGGGGTCGGCTGCTCCCTGCCTGCAGAGGGCTCTCCCCCGG
  CCCTCGAACCAGCCTTCTCCAAGATTCTGAACCGTCTCAGCCGCCCAAAGTCATCAGGACAGGGCGGCGC
  AAGAAATGCGGCTCTTAAAGACCTCGGCGCTTTAATAGAAGCCGCCGAAGGTGACAGATTCTTTGAGGGG
  AGTGGGAGTGGGGGATCCCTCCGGGGGATGCCCGAGATCCTGGGGCAGGTGGTGAGAGCCCTGGAGAAGT
  TCGCAGCACCCGAGGAGAAGGCAGATGGGGTCGAAGAGCATCCCGAAGTGCCCGAGAAGGCGACTGAAGT
  TGGCTCACTGTTTCTAAAGCTGTTGGGGAAGGTTGAAGCTGCCAAGAGTTCTCCCGACTGCCCTGCGTGG
  AAGACAGGCCTCCGTCACATGTCAGGACCCGTGTATATTTTTGCCATCACTCACAGATTGAAGCAGCCAT
  GGACCAGTCCAGCATCTCAGCATGTTGCTGGTGAGGTGCTGTCTTTATTGCTTAGAGTTACTGAGTGCAG
  TTCTGTGGCAGGATTCCTATGCGGAGAAAATGAAGACGATCGAGGGAGATTTGCTGTGGTCTTGGGGCTT
  CTTAAGCCCCATTTGAATAAGGAAACCTGGAAGAAGAATCCTGCTGTCAAACATGTTTTCTCGTGGACTC
  TCCAACAAGTCACTCAGCCCTGGCTGAATCAGCATCTAGAAAAGATACTTCCTCCGTCTTTGCTCATCTC
  AGATGACTATCAGACAGAGAATAAAATCCTGGGTGTCCAGTGCCTCCATCACATTGTGGTTACTGTGCCA
  GCTGCGGACTTGCTGCAGTATAACAGAGCCCAGGTCCTGTACCACGCCCTCTTCAATCACCTGTACATGC
  CTGAGCACCACCTCATTCAGGCTGTGCTCCTGTGCCTCCTAGACTTGTTCCCTGTCCTAGAGAAGGCTCT
  GCACTGGAAGGGAGACACAGCTCGAGTCACCACACACTGTCATGAAGTGTTACAGCTGATCCTCACGCAC
  ATGGAACCAGAGCACCGCCTCCTCCTACGGAGGACCTATGCCAGACACCTGCCAGCCTTCGTGAAGAGGT
  TGGGGATCCTAACTGTCCGGCACTTGAAGAGGCTGGAGCAAGTTATCCTTGGTTATCTGGAGGTTTATGA
  TGAACCAGAAGATGAAACTAGGTTGAAGATCCTGGAAACCCTGAAACTTGTCATGCAGTATACTTGGCCC
  AGAATTCCCTGCAGAGTTGTGGTCTTACTGAAGGCTCTCCTGAAACTCATATGTGATATATCGAGGGATA
  CGATCCCCACAACCGAGGCCGCTAAGAGTACAATGCTCCAGGAAGCCACAGACTGCCTGATTCTCCTGGA
  CCACTGTTCCCAGGGGCAGGTGAAGGGTCTTCTGGCTAAAATTGCAGTATCCTGTGAAGACAGCACAGTG
  GTGAGCTGCATCAGGAAGGTGCAGCAGGGCTCTGCAGATTCTCCTGGCGATGACACTGAAGGGCCTTGTC
  GAAGGCATGGATGGGGAAGTTGTAGATGTCGCCATACTGAAGAAGAAACACAGACCACCTTTAACTATGC
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199988
• Insert Size: 1614 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199988.1, NP_001186917.1
• RefSeq Size: 4978 bp
• RefSeq ORF: 1614 bp
• Locus ID: 234138
• Cytogenetics: 8 A3
• Gene Summary: Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region and includes a different terminal exon, compared to variant 2. The resulting protein (isoform b) is longer and has a distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.