Elf2 (NM_001291063) Mouse Untagged Clone

CAT#: MC228208

Elf2 (untagged) - Mouse E74-like factor 2 (Elf2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Elf2
• Synonyms: 2610036A20Rik; A230104O07Rik; AW111824; BB183398; EU32; NERF; NERF-1A; NERF-1B; NERF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228208 representing NM_001291063
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAG
  TTCACAGCAGTAATGCTCACTGTACGGATAAGACCATTGAAGCTGCTGAAGCCCTGCTTCATATGGAATC
  TCCTACCTGCTTGAGGGATTCAAGAAGTCCTGTGGAAGTGTTTGTCCCTCCTTGTATATCAACTCCAGAG
  TTTATCCATGCTGCGATGAGGCCGGATGTCATCACAGAGACTGTAGTGGAGGTGTCCACAGAAGAGTCTG
  AGCCGATGGATGCCTCGCCTATCCCTACTTCACCAGACAGCCACGAACCAATGAAAAAGAAAAAAGTTGG
  CCGTAAACCAAAGACCCAGCAGTCACCAGTTTCCAATGGGTCTCCTGAGTTAGGAATCAAGAAGAAAGCC
  AGAGAAGGAAAAGGAAACACAACCTATCTGTGGGAGTTTCTTTTAGATTTGCTTCAAGATAAAAATACTT
  GTCCCAGGTATATTAAATGGACTCAGAGAGAAAAGGGCATATTCAAACTTGTGGACTCAAAGGCTGTCTC
  GAAGCTCTGGGGGAAGCATAAGAACAAACCAGATATGAACTATGAAACCATGGGGCGAGCGTTAAGATAT
  TACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTTGTATATCAGTTCAAGGACATGCCCA
  AAAACATAGTGGTCATTGATGATGACAAAAGTGAGACCTGCCCTGAAGACTTGGCAGCAGCTGCTGATGA
  CAAGTCTTTAGAACGAGTGTCACTATCTGCAGAAAGTCTCCTGAAGGCAGCAACTGCCGTCCGAGGTGGG
  AAAAACTCATCTCCTCTGAACTGCTCCAGAGCAGAGAAGGGTGTGGCTAGAGTTGTGAACATCACCTCCC
  CTACTCACGACGGTTCCTCCAGGTCTCCTACTACCACTGCACCTGTGTCAGCAGCAGCAGCTCCAAGGAC
  AGTTCGTGTGGCAATGCAAGTCCCTGTGGTAATGACCTCACTGGGCCAAAAGATTTCAGCGGTGGCAGTT
  CAGTCAGTCAATGCAGGCACAGGCTCGCCGTTAATAACCAGCACCAGTCCAGCCTCGGCCAGCTCTCCAA
  AAGTAGTCATCCAGACAGTCCCGACCGTGATGCCGGCCTCCACTGAAAATGGAGACAGAATCACCATGCA
  GCCTGCCAAGATTATCACCATCCCCGCCACCCAGCTCGCACAGTGTCAACTCCAGGCAAAGTCAAACCTG
  ACGGGGTCAGGAAGCATTAACATTGTTGGAACCCCACTGGCTGTGAGGGCACTCACCCCTGTTTCAATAG
  CCCACGGTACACCTGTAATGAGACTGTCTGTGCCTGCTCAGCAGGCTTCTGGCCAGACTCCTCCTCGAGT
  TATCAGTGCGCTCCTAAAAGGGCCGGAGGGGAAATCAGAAGCCAAAAAGCAGGAACACGATGTGAAAACT
  TTGCAGCTGGTAGAAGAGAAGGGGGCAGACGGCAATAAGACAGTAACCCACGTAGTGGTCGTCAGTGCGC
  CGTCTGCTATCGCCCTTCCTGTGACTATGAAAACCGAAGGGCTAGTGACGTGTGAGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291063
• Insert Size: 1602 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291063.1, NP_001277992.1
• RefSeq Size: 5495 bp
• RefSeq ORF: 1602 bp
• Locus ID: 69257
• UniProt ID: Q9JHC9
• Cytogenetics: 3 C
• Gene Summary: Probably transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate 5' structure compared to variant 1. The encoded isoform (4) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.