Mgrn1 (NM_001252437) Mouse Untagged Clone

CAT#: MC228205

Mgrn1 (untagged) - Mouse mahogunin, ring finger 1 (Mgrn1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mgrn1
• Synonyms: 2610042J20Rik; md; mKIAA0544; nc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228205 representing NM_001252437
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCATCCTGAGTCGCCGCATCGCGGGCGTAGAGGACATCGACATACAGGCCAACTCGGCCTATC
  GCTACCCTCCGAAGTCCGCAGGAAACTACTTTGCTTCGCATTTTTTCATGGGAGGAGAGAAATTTGACAC
  TCCCCATCCTGAAGGTTACCTCTTTGGAGAGAACATGGACCTGAACTTCCTGGGCAGTCGTCCAGTCCAG
  TTTCCCTATGTCACCCCTGCCCCCCATGAGCCAGTGAAGACACTGAGGAGCCTGGTGAACATTCGCAAAG
  ACTCTCTCCGGCTTGTGAGGTACAAAGAAGACGCAGACAGCCCCACGGAGGACGGTGAAAAGCCCCGCGT
  GCTCTACAGTCTGGAATTCACCTTTGATGCCGATGCCCGTGTGGCTATCACCATCTATTGTCAGGCGGTA
  GAGGAGCTGGTGAATGGCGTGGCAGTGTACAGCTGTAAGAACCCTTCTCTGCAATCTGAGACCGTCCACT
  ACAAGAGAGGCGTTAGCCAGCAGTTCTCCCTGCCTTCTTTCAAGATTGACTTCTCCGAGTGGAAGGATGA
  TGAGCTGAACTTTGACCTGGACCGGGGTGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGGGAT
  GTTGTGGAAGTGACTGGCCACGCTCATGTGCTCCTGGCTGCCTTTGAGAAGCATGTGGATGGCAGCTTCT
  CCGTGAAGCCATTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTGCTGCAGGAGATCTACGGCAT
  CGAGAACAAGAACAACCAGGAGACTAAGCCTTCTGATGATGAGAACAGTGACAACAGCAGTGAGTGTGTG
  GTGTGCCTGTCAGACCTGCGGGACACACTGATCCTGCCCTGCCGACACCTGTGCCTCTGCACTTCCTGTG
  CTGACACACTGCGGTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCGTTCCGAGCCCTTCTGCAGAT
  CCGGGCTGTGCGGAAGAAGCCAGGGGCCCTGTCTCCCATCTCCTTCAGCCCTGTCTTGGCCCAAAGTGTG
  GACCACGATGAGCACTCTAGTTCTGACAGTATCCCACCTGGCTATGAGCCCATCTCCTTGCTTGAGGCAC
  TCAATGGTCTACGAGCTGTCTCCCCGGCTATCCCATCAGCTCCCCTCTATGAGGAAATCACCTACTCAGG
  CATCTCGGACGGTCTTTCCCAGGCCAGTTGTCCCCTTGCTGGACTTGATCGAATCATGGAAAGTGGCCTA
  CAGAAGGGCAAGACACAGAGCAAGTCTCCAGACAGCACCCTGCGGTCTCCATCATTCCCCATCCATGAGG
  AGGACGAGGAGAAGCTCTCAGAGGACTCGGATGCTCCTCTCCCACCGAGCGGTGTGGAGCTGGTACTGCG
  GGAGAGCAGCTCCCCTGAGAGTTTCGGAACAGAGGAGGGAGATGAGCCGTCCCTAAAGCAAGGGAGCCGA
  GTACCCTCTATTGATGATGTCCTGCAAGATGGCAGTCCGCAGCATCATGGTTGCAGCCAGCCAGTCCCTC
  CTGCTGACATCTACCTACCAGCCCTGGGTCCTGAGTCGTGCTCTGTTGGTATAGAGGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252437
• Insert Size: 1602 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252437.1, NP_001239366.1
• RefSeq Size: 3293 bp
• RefSeq ORF: 1602 bp
• Locus ID: 17237
• UniProt ID: Q9D074
• Cytogenetics: 16 2.48 cM
• Gene Summary: E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination (By similarity). Acts also as a negative regulator of hedgehog signaling (PubMed:29290584).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.