Pkm (NM_001253883) Mouse Untagged Clone

CAT#: MC228197

Pkm (untagged) - Mouse pyruvate kinase, muscle (Pkm), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pkm
• Synonyms: AA414905; AL024370; AL024424; Pk-2; Pk-3; Pk3; Pkm2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228197 representing NM_001253883
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAAGCCACACAGTGAAGCAGGGACTGCCTTCATTCAGACCCAGCAGCTCCATGCAGCCATGGCTG
  ACACCTTCCTGGAACACATGTGCCGCCTGGACATTGACTCTGCCCCCATCACGGCCCGCAACACTGGCAT
  CATTTGTACCATTGGGCCTGCTTCCCGATCTGTGGAGATGCTGAAGGAGATGATTAAGTCTGGAATGAAT
  GTGGCTCGGCTGAATTTCTCTCATGGAACCCATGAGTACCATGCAGAGACCATCAAGAATGTCCGTGAAG
  CCACAGAAAGCTTTGCATCTGATCCCATTCTCTACCGTCCTGTTGCGGTGGCTCTGGATACAAAGGGACC
  TGAGATCCGGACTGGACTCATCAAGGGCAGCGGCACCGCTGAGGTGGAGCTGAAGAAGGGAGCCACTCTG
  AAGATCACCCTGGACAACGCTTACATGGAGAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACA
  TCTGCAAGGTGGTGGAGGTGGGCAGCAAGATCTACGTGGACGATGGGCTCATCTCACTGCAGGTGAAGGA
  GAAAGGCGCTGACTTCCTGGTGACGGAGGTGGAGAATGGTGGCTCCTTGGGCAGCAAGAAGGGCGTGAAC
  CTGCCGGGCGCTGCTGTGGATCTCCCCGCTGTGTCGGAAAAGGACATCCAGGACCTGAAGTTTGGGGTGG
  AGCAGGATGTGGACATGGTGTTTGCATCTTTCATCCGCAAGGCAGCCGACGTGCATGAAGTCAGGAAGGT
  GCTGGGAGAGAAGGGCAAGAACATCAAGATCATCAGCAAAATCGAGAACCATGAAGGCGTCCGCAGGTTT
  GATGAGATCTTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGACCTGGGCATTGAGATTCCTGCAG
  AGAAGGTCTTCCTGGCTCAGAAGATGATGATCGGGCGATGCAACCGAGCTGGGAAGCCTGTCATCTGTGC
  CACACAGATGCTGGAGAGCATGATCAAGAAGCCACGCCCCACCCGTGCTGAAGGCAGTGATGTGGCCAAT
  GCAGTCCTGGATGGAGCAGACTGCATCATGCTGTCTGGAGAAACAGCCAAGGGGGACTACCCTCTGGAGG
  CTGTTCGCATGCAGCACCTGATAGCTCGGGAGGCTGAGGCAGCCATGTTCCACCGTCTGCTGTTTGAAGA
  GCTTGTGCGAGCCTCCAGTCACTCCACAGACCTCATGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCT
  TATAAGTGTTTAGCAGCAGCTTTGATAGTTCTCACGGAGTCTGGCAGGAGTGCTCACCAAGTGGCCAGGT
  ACCGCCCTCGGGCTCCTATCATTGCCGTGACTCGAAATCCCCAGACTGCTCGCCAGGCCCATCTGTACCG
  TGGCATCTTCCCTGTGCTGTGTAAGGATGCCGTGCTGAATGCCTGGGCTGAGGATGTCGACCTTCGTGTA
  AACTTGGCCATGGATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCG
  GGTGGCGCCCTGGCTCTGGATTCACCAACACCATGCGTGTAGTGCCTGTACCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253883
• Insert Size: 1596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253883.1, NP_001240812.1
• RefSeq Size: 2432 bp
• RefSeq ORF: 1596 bp
• Locus ID: 18746
• UniProt ID: P52480
• Cytogenetics: 9 32.03 cM
• Gene Summary: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (By similarity). Stimulates POU5F1-mediated transcriptional activation (By similarity). Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (PubMed:29996098).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform M1. Isoforms M1 and M2 are identical in length but have different sequences.