Traf6 (NM_001303273) Mouse Untagged Clone

CAT#: MC228188

Traf6 (untagged) - Mouse TNF receptor-associated factor 6 (Traf6), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Traf6
• Synonyms: 2310003F17Rik; AI851288; C630032O20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228188 representing NM_001303273
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTCTTAAACTGTGAGAACAGCTGCGGGTCCAGCCAGTCGTCCAGTGACTGCTGCGCTGCCATGG
  CCGCCTCCTGCAGCGCTGCAGTGAAAGATGACAGCGTGAGTGGCTCTGCCAGCACCGGGAACCTCTCCAG
  CTCCTTCATGGAGGAGATCCAGGGCTACGATGTGGAGTTTGACCCACCTCTGGAGAGCAAGTATGAGTGT
  CCCATCTGCTTGATGGCTTTACGGGAAGCAGTGCAAACACCATGTGGCCACAGGTTCTGCAAAGCCTGCA
  TCATCAAATCCATAAGGGATGCAGGGCACAAGTGCCCAGTTGACAATGAAATACTGCTGGAAAATCAACT
  GTTTCCCGACAATTTTGCAAAGCGAGAGATTCTTTCCCTGACGGTAAAGTGCCCAAATAAAGGCTGTTTG
  CAAAAGATGGAACTGAGACATCTCGAGGATCATCAAGTACATTGTGAATTTGCTCTAGTGAATTGTCCCC
  AGTGCCAACGTCCTTTCCAGAAGTGCCAGGTTAATACACACATTATTGAGGATTGTCCCAGGAGGCAGGT
  TTCTTGTGTAAACTGTGCTGTGTCCATGGCATATGAAGAGAAAGAGATCCATGATCAAAGCTGTCCTCTG
  GCAAATATCATCTGTGAATACTGTGGTACAATCCTCATCAGAGAACAGATGCCTAATCATTATGATCTGG
  ACTGCCCAACAGCTCCAATCCCTTGCACATTCAGTGTTTTTGGCTGTCATGAAAAGATGCAGAGGAATCA
  CTTGGCACGACACTTGCAAGAGAATACCCAGTTGCACATGAGACTGTTGGCCCAGGCTGTTCATAATGTT
  AACCTTGCTTTGCGTCCGTGCGATGCCGCCTCTCCATCCCGGGGATGTCGTCCAGAGGACCCAAATTATG
  AGGAAACTATCAAACAGTTGGAGAGTCGCCTAGTAAGACAGGACCATCAGATCCGGGAGCTGACTGCCAA
  AATGGAAACTCAGAGTATGTACGTGGGCGAGCTCAAACGGACCATTCGGACCCTGGAGGACAAGGTTGCC
  GAAATGGAAGCACAGCAGTGTAACGGGATCTACATTTGGAAGATTGGCAACTTTGGGATGCACTTGAAAT
  CCCAAGAAGAGGAAAGACCTGTTGTCATCCATAGCCCTGGATTCTACACAGGCAGACCTGGGTACAAGCT
  GTGCATGCGCCTGCATCTTCAGTTACCGACAGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCAC
  ACAATGCAAGGAGAATATGACAGCCACCTCCCCTGGCCCTTCCAGGGTACAATACGCCTTACAATTCTCG
  ACCAGTCTGAAGCACTTATAAGGCAAAACCACGAAGAGGTCATGGACGCCAAACCAGAACTGCTTGCCTT
  TCAGCGACCCACAATCCCACGGAACCCCAAAGGTTTTGGCTATGTAACATTTATGCACCTGGAAGCCTTA
  AGACAGGGAACCTTCATTAAGGATGATACATTACTAGTGCGCTGTGAAGTCTCTACCCGCTTTGACATGG
  GTGGCCTTCGGAAGGAGGGTTTCCAGCCACGAAGTACTGATGCGGGGGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001303273
• Insert Size: 1593 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303273.1, NP_001290202.1
• RefSeq Size: 5985 bp
• RefSeq ORF: 1593 bp
• Locus ID: 22034
• UniProt ID: P70196
• Cytogenetics: 2 E2
• Gene Summary: This gene encodes a member of the TNF receptor associated factor (TRAF) family of adaptor proteins that mediate signaling events from members of the TNF receptor and Toll/IL-1 receptor families to activate transcription factors such as NF-kappa-B and AP-1. The product of this gene is essential for perinatal and postnatal survival. Mice deficient in this protein exhibit osteopetrosis and defective in development of epidermal appendixes, normal B cell differentiation, lymph node organogenesis, interleukin-1 signaling, lipopolysaccharide signaling and neural tube closure. This protein possesses ubiquitin ligase activity. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.