Fbxw11 (NM_001271348) Mouse Untagged Clone

CAT#: MC228186

Fbxw11 (untagged) - Mouse F-box and WD-40 domain protein 11 (Fbxw11), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxw11
• Synonyms: 2310065A07Rik; AA536858; BTRC2; BTRCP2; Fbxw1b; HOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228186 representing NM_001271348
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGACTCGGTGATTGAAGACAAGACCATCGAGCTCATGAACACTTCAGTTATGGAAGATCAAA
  ATGAAGATGAGTCCCCAAAGAAAAGTGCTCTTTGGCAGATCAGTAATGGAACGTCATCTGTGATTGTCTC
  CAGAAAGAGGCCGTCAGAGGGGAACTACCAGAAAGAAAAGGACTTGTGCATTAAGTACTTTGACCAGTGG
  TCTGAATCAGATCAGGTGGAATTTGTGGAGCATCTTATCTCACGGATGTGTCATTATCAGCATGGACATA
  TTAACTCTTACCTGAAGCCCATGTTGCAGCGGGACTTTATCACTGCTTTACCAGAGCAAGGCTTAGATCA
  CATAGCAGAAAACATTCTCTCCTACCTGGATGCCAGGTCTCTGTGTGCAGCAGAGCTGGTGTGTAAAGAA
  TGGCAGCGAGTGATCTCAGAAGGGATGCTTTGGAAGAAGCTGATTGAGAGGATGGTGCGCACCGACCCTC
  TCTGGAAGGGACTCTCAGAAAGAAGAGGCTGGGATCAGTACCTGTTTAAAAACAGACCTACAGATGGCCC
  TCCCAACTCATTTTATAGATCATTATACCCAAAGATTATCCAGGACATAGAGACCATAGAATCCAACTGG
  CGGTGTGGACGACACAACTTGCAGAGGATCCAGTGCCGCTCTGAAAATAGTAAGGGTGTCTACTGTTTGC
  AATATGATGATGACAAAATTATCAGTGGCCTCCGGGACAACTCTATCAAGATCTGGGATAAAAGCAGCTT
  GGAATGTTTGAAAGTGCTAACGGGCCACACAGGCTCTGTCCTCTGCCTCCAGTATGATGAGCGAGTCATT
  GTAACTGGTTCTTCAGACTCCACGGTGAGAGTCTGGGATGTGAACACTGGTGAGGTGCTCAACACACTCA
  TCCACCACAATGAAGCCGTACTGCACTTACGCTTCAGCAATGGACTGATGGTGACTTGTTCCAAGGACCG
  TTCCATTGCCGTGTGGGACATGGCTTCTGCCACCGATATCACTTTACGCCGTGTTCTGGTTGGCCACCGT
  GCTGCTGTCAATGTAGTAGACTTTGATGATAAATACATCGTGTCTGCTTCAGGAGACAGGACCATTAAAG
  TGTGGAGCACGAGCACATGTGAGTTTGTCCGCACTCTGAATGGGCACAAGCGAGGCATCGCCTGTCTGCA
  GTACCGCGACCGGCTTGTTGTTAGTGGATCATCAGATAATACCATCCGGTTATGGGATATTGAATGTGGT
  GCCTGTTTAAGAGTCCTAGAGGGGCACGAAGAATTGGTCCGGTGCATCCGTTTTGATAACAAGAGGATTG
  TCAGTGGCGCCTATGATGGGAAGATTAAAGTCTGGGACTTGCAGGCTGCTCTTGACCCTCGGGCCCCAGC
  AAGCACATTGTGTCTGCGCACCTTGGTGGAACACTCTGGACGTGTGTTTCGGCTGCAGTTTGATGAGTTT
  CAGATCATCAGCAGCTCCCATGATGACACTATTTTGATTTGGGATTTCTTAAATGTGCCTCCCAGTGCCC
  AGAATGAGACCCGCTCTCCCTCCAGAACCTACACCTATATCTCCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271348
• Insert Size: 1590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271348.1, NP_001258277.1
• RefSeq Size: 4237 bp
• RefSeq ORF: 1590 bp
• Locus ID: 103583
• UniProt ID: Q5SRY7
• Cytogenetics: 11 A4
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. SCF(FBXW11) mediates the ubiquitination of phosphorylated CTNNB1 and participates in Wnt signaling. SCF(FBXW11) mediates the ubiquitination of phosphorylated NFKBIA, which degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. SCF(FBXW11) mediates the ubiquitination of IFNAR1. SCF(FBXW11) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (By similarity). Involved in the oxidative stress-induced a ubiquitin-mediated decrease in RCAN1. Mediates the degradation of CDC25A induced by ionizing radiation in cells progressing through S phase and thus may function in the intra-S-phase checkpoint. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and phosphorylated PER2. SCF(FBXW11) mediates the ubiquitination of CYTH1, and probably CYTH2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (c) is shorter than isoform a.