Gabrq (NM_001290435) Mouse Untagged Clone

CAT#: MC228174

Gabrq (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit theta (Gabrq), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabrq
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228174 representing NM_001290435
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCCGAGGTATGCTGCGAGCCGCTGCGCTCCTGCTGCTCATTAGGACTTGGCTGGCAGAGAGTA
  ACGGCCCCAGTCCCACCCCGAAATTCCACTTCGAGCTCTCCTCCAGCACGCCTGAAGTCATTCTGGATCT
  CTTCAACTGCAAAAACTGTGCAAATGAAGCTGTGGTTCAAAAGATTTTGGACAGGGTGCTGTCAACATAT
  GATGTCCGACTTAGACCAAATTTTGGAGGAGCTCCTGTGCCTGTGTCAGTATCTATCTATGTGTCCAGTA
  TTGAACAGATCTCAGAAATAAATATGGACTATACAATCACAATGTTTTTGCATCAGACCTGGAAGGACAC
  ACGCTTAGCTTACTATGAAACCAACCTAAACCTGACCCTGGATTATCGGATGCATGAGAAGCTCTGGGTT
  CCTGACTGCTACTTTGTGAATAGCAAAGATGCCTTTGTGCATGATGTAACTGTGGAGAATCGTGTATTTC
  AACTTCACCCAGATGGGACAGTGCGATATGGCATCCGACTTACCACAACAGCAGCCTGTTCCCTGGATCT
  GCAAAAATTCCCTATGGACAAACAATCCTGCAAGCTGGAGGTGGAGAGCTATGGCTACACTGTCGAAGAC
  ATTGTGTTATCCTGGGAAGATGATAATGCCATCCACATTACTGATGGGCTGCATATCCCTCAGTACACGT
  ACCTGGGGAGAACAATTACCAGCAAGGAAGTGTACTTCTACACAGGCTCCTACATGCGCCTGATAGTGAA
  GTTCCAGGTTCAGAGGGAAGTCCGCAGTTACCTTGTGCAGGTCTATTGGCCCACGGTCCTCACCACCATT
  CTCTCCTGGATATCATTTTGGATGAACTATGATTCCTCTGCAGCCAGGGTAACTATTGGACTAACTTCGA
  TCCTTGTCCTGACTACCATCGACTCACATATGCGGGATAAACTCCCCCATATTTCCTGTATCAAAGCTAT
  TGACATCTACATCCTTGTATGCCTATTCTTCGTTTTCCTGTCACTGCTGGAGTATGTCTACATCAACTAC
  CTTTTCTTCAGCCAAGTACCTCGGCGTAATCACAGAAGATGCAGGAAACCCAGGAGAGTTGTTGCCCGAT
  ACCGCTACCAAGAAGTGGTGGTAGCAAATGTGCAGGATGGCCTGATTAATGTGGAAGACAGAGTAGAAGA
  CAGAGCTGGTCCCCTTCCTGACAGCCCCATGCAGGCTCATCTGGCAAGCCAGGAAAGTCTAGGATCCTTG
  GTGTTCACATCAGAGCAGGCCCAGCTGGCTACCTCAGAAAGTCTCAGTCTACTCTCTTCTGCCTCAAGCC
  AGACTCAATTGGCCACTGGAGAAAGCCTGAGTGATCTGCCCTCCACCTCTGAGCAAACAGTGCCTGAGTG
  TACCATTCACTTTCATGGCTTTCTTACTAATGACAGTATTATCCCCATCAAAATCCACAGCCGATCTGAT
  GCCTGTGATGATGAAGACTCCGAAGAAAGCCTAAGCTCTGAGGAGAGCCATGGCCATGGAAGCAGCCACA
  CTGGAAGGCTCAAGCTCCAGATTAGCCAGAGTGCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290435
• Insert Size: 1578 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290435.1, NP_001277364.1
• RefSeq Size: 4943 bp
• RefSeq ORF: 1578 bp
• Locus ID: 57249
• Cytogenetics: X A7.3
• Gene Summary: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (a).