Wdr76 (NM_001290986) Mouse Untagged Clone

CAT#: MC228167

Wdr76 (untagged) - Mouse WD repeat domain 76 (Wdr76), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdr76
• Synonyms: 5830411K18Rik; 6030457D09; AA517836
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228167 representing NM_001290986
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCCTTATCTTCCAAGGCAGATTCCCTTCTGCTGAAATCATCCGTGGATGCTTATACTGAAAGTA
  CCAGGCTGGGACCCAAGAGGACCTCGGATTCAGCGACTCTCAGTGTGGACGCGGAAAGCAGCGACGAAGA
  CAGTGCACCAGGCCTGGATGATTTTTCAGGATTGTCACCTTATGAAAGGAAGAGACTGAGGAACATCAGA
  GAAAATGCAAACTTTTTTGCTTCCCTTCAGTTAGCTGAGTCGGCTGCACGGCTCCGTGGGATGATAAAGA
  AGCGCGAGTCACCTGAGTCAAAGAGAAAGAGGCCTAAGAAGAAAGAAAATGAAATTGGATGTCGAAGGTC
  CATGCGGTTGTTGAAAGTAGACCCTTTGGGAGTTTCATTACCAGCATCTCCAACACAGCCAACATTAGTA
  GAAGAAGAAGAAAATCCTTTGTTACCTCCTGGACCCTTAGAAATGATTCCTGAAAACCAAGATGACAGCA
  GTGAACTGTTGAAAGCATCTTTGAAGACATGGGCAGAGATGAGCCAGACAAGTAATGAGAAGACCAAGAA
  GGGGCTGTCTAGTATTAAAAGCTATAAAGCCAATTTAAGTGGCATGGTCATCAGTGAGGCTACTGTTCGT
  AAGGTGACCAAAGGAGCAATCTCCTCCGTGGCCCTCCACCCATCAGAAGTGAGAACCTTGGTAGCAGCTG
  GGGCCAAGTCTGGACAAATTGGACTTTGGGATTTGACCCAGCAGAGTGAAGATGCGATGTACGTTTTCTA
  TGCTCACAGTCGGTATGTTAGCTGCCTCTCCTTCTCACCCACCAACCCAGCCCATCTACTGTCCCTGAGC
  TATGACGGCACACTGCGATGTGGTGATTTTTCCAGCGCTGTGTTTGAGGAGGTGTACAGGAATGAGGGAA
  ACAGCCCGTCCTCCTTTGACTTCTTGAACGATTCCTCCAGTCTGCTAGTGGGACACTGGGATGGACATTT
  GTCACTGGTAGATAGACGGACACCTGGAACTTCTTATGAGAAGTTTTTTAATTCATCTTTGGAAAAAATA
  AGAACTGTTCATGTCCACCCACTATCCAGACAGTATTTTGTCACAGCAGGATTGAGGGACGTTCATGTTT
  ACGATGCCAGGTTCCTGAAGTCCAGGGGAAGCCAGCCTCTGATTTCCTTGACTGAGCACTCGAAAAGCAT
  TGCCTCTGCATATTTCTCCCCCGTTACTGGTAACAGAGTGGTGACTACATGTGCTGACTGTAAGCTCAGG
  GTCTTTGACAGCAGCTCCATATCTTCCCAGCTTCCTCTCCTCAGTACCATCAGGCACAACACTGTCACTG
  GGAGGTGGCTAACGAGGTTCCAGGCTGTGTGGGACCCTAAACAAGAAGATTGCTTCATAGTTGGTAGCAT
  GGACCACCCACGACGGGTGGAAGTCTTCCACGAGTCGGGAAAGAATGTGCATTCTCTTTGGGGAGAATGT
  CTTGTATCTGTGTGTTCCCTCAGTGCTGTGCATCCTACGCGATATATCCTGGCTGGAGGTAATTCCAGTG
  GCAAGTTACATGTTTTTATGCATCAAGAAACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290986
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290986.1, NP_001277915.1
• RefSeq Size: 4246 bp
• RefSeq ORF: 1575 bp
• Locus ID: 241627
• UniProt ID: A6PWY4
• Cytogenetics: 2 E5
• Gene Summary: Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a).