Cyp4f14 (NM_001204334) Mouse Untagged Clone

CAT#: MC228164

Cyp4f14 (untagged) - Mouse cytochrome P450, family 4, subfamily f, polypeptide 14 (Cyp4f14), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp4f14
• Synonyms: 1300014O15Rik; cyp4f-14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228164 representing NM_001204334
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAGCTGAGCCTGTCCTGGTTGGGACTGGGGCCCGAGGTGGCCTTCCCATGGAAGACCCTGCTAC
  TGCTTGGGGCCTCCTGGATCCTGGCCCGGATTCTGATCCAGATCTATGCTGCCTATAGGAACTATCGTCA
  CCTCCATGGCTTCCCTCAGCCCCCCAAACGGAACTGGCTTATGGGTCACGTGGGCATGGTAACTCCTACA
  GAGCAGGGCTTGAAGGAATTGACTCGTTTGGTGGGTACCTACCCCCAAGGTTTTTTGATGTGGATTGGAC
  CAATGGTCCCTGTTATTACTCTATGCCATTCTGACATCGTCCGATCTATCCTCAATGCCTCAGCCGCTGT
  TGCACTCAAGGATGTGATATTCTACAGCATCCTAAAGCCTTGGCTGGGGGATGGGCTCTTGGTGAGTGCT
  GGTGACAAGTGGAGCCGCCATCGTCGCATGCTGACACCCGCTTTCCATTTCAACATCCTGAAGCCCTATG
  TGAAGATTTTCAACGATAGCACCAACATCATGCACGCCAAGTGGCAACGGTTGATTTCAGATGGCAGTGC
  CCGTCTGGACATGTTTGAGCACGTCAGCCTGATGACTCTGGATAGTCTGCAGAAATGTGTCTTCAGCTTT
  GACAGCAACTGTCAGGAGAAGTCCAGTGAATATATTGCTGCCATCCTGGAGCTCAGTGCCCTAGTGGCCA
  AAAGGCACCAGCAGCCTTTGATGTTCATGGACCTGCTGTATAATCTCACCCCTGATGGTATGCGCTTCCG
  TAAGGCCTGCAACGTGGTGCATGAATTCACTGATGCTGTCATCCGGGAGCGGCACCGCACCCTCCCAGAT
  CAGGGTTTGGATGACTTCCTTAAGTCTAAGGCCAAGTCCAAGACTTTGGATTTCATTGATGTGCTGCTGC
  TGAGCAAGGATGAAGATGGAAAGGAGCTGTCAGATGAGGACATCCGAGCAGAGGCTGACACCTTCATGTT
  TGAGGGCCATGACACGACAGCCAGTGGACTCTCCTGGATCCTCTACAACCTGGCAAGGCACCCAGAATAC
  CAGGAGCGCTGCCGGCAGGAAGTTCAGGAGCTGCTGAGGGGCCGAGAGCCTGAGGAGATTGAATGGGACG
  ACCTGGCCCAGCTACCCTTCCTGACCATGTGCATCAAGGAGAGTCTGCGACTGCATCCCCCAGTCACGGT
  CATCTCCCGATGCTGCACCCAGGACATTCTGCTCCCAGATGGCCGGACCATTCCCAAAGGTATCATCTGT
  CTCATCAGCATCTTTGGAATTCATCACAACCCATCCGTGTGGCCAGACCCTGAGGTCTATGACCCCTTCC
  GCTTTGACCCTGAAAACATCAAAGACAGCTCACCTCTGGCATTTATTCCCTTCTCGGCGGGACCCAGGAA
  CTGCATAGGACAGACTTTCGCCATGAGTGAGATGAAGGTGGCACTGGCGCTGACGCTGCTGCGCTTCCGA
  CTCCTACCGGATGACAAGGAGCCGCGCAGGCAGCCAGAGCTGATCCTGCGCGCAGAGGGCGGGCTGTGGC
  TGCGGGTGGAGCCGCTGAGCGCGGGCGCTCATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204334
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204334.1, NP_001191263.1
• RefSeq Size: 2051 bp
• RefSeq ORF: 1575 bp
• Locus ID: 64385
• UniProt ID: Q9EP75
• Cytogenetics: 17 B1
• Gene Summary: Cytochromes P450 are a group of heme-thiolate monooxygenases. Catalyzes the omega-hydroxylation of LTB4 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3, 4 and 5 encode the same protein.