Ppil2 (NM_001252445) Mouse Untagged Clone

CAT#: MC228144

Ppil2 (untagged) - Mouse peptidylprolyl isomerase (cyclophilin)-like 2 (Ppil2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppil2
• Synonyms: 0610009L05Rik; 1700016N17Rik; 4921520K19Rik; 4930511F14Rik; AA589416; C130078A06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228144 representing NM_001252445
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGCGACAGCACCAGAAGGACAAGATGTATATCACTTGTGCTGAGTACACTCATTTCTATGGTG
  GCAGGAAGCCAGATATCTCACAGACAAGTTTTCGCCGCTTACCTTTTGACCACTGCAGTCTCTCTCTCCA
  GCCTTTTGTCTACCCAGTCTGCACCCCAGAAGGTGTCGTCTTTGACTTGCTGAACATTGTTCCCTGGCTT
  AAGAAGTATGGGACGAATCCCAGCACTGGAGAGAAACTTGATGGGAAGTCCTTGATCAAGCTGAACTTCG
  CAAAGAACAGCGAAGGGCAGTACCACTGTCCAGTGCTGTATTCCGTGTTCACTGACAACACCCATATTGT
  GGCCATCAGGACAACTGGCAATGTCTACACCTATGAGGCAGTGGAGCAGCTAAACATCAAGGCCAAGAAC
  TTGCGGGATCTGTTGACTGATGAGCCCTTTTCCAGGCAAGACATCATCACCCTGCAGGACCCCACCAATT
  TGGACAAATTCAATGTTAGCAACTTCTTCCATGTGAAGAATAACATGAGAATAATAGACCCAGATGAGGA
  AAAGGCCAAACAAGACCCATCTTATTATTTGAAAAACACAAATTCGGAGACGAGAGAGACGCTACAGGAG
  CTCTACAAAGAGTTCAAAGGAGATGAGATTTTAGCAGCTACCATGAGGCCACCTGAGAAGAAGAAGGTGG
  ACCAACTGAATGCGGCCCACTACTCCACAGGGAAGGTCAGTGCATCCTTCACCTCTACTGCCATGGTGCC
  CGAGACCACGCATGAAGCAGCTGTCATTGATGAAGATGTACTGCGCTACCAGTTTGTGAAGAAAAAGGGC
  TATGTAAGGCTTCACACCAACAAGGGCGACCTTAACTTAGAGCTGCACTGTGACCTGACACCAAAAACCT
  GTGAAAACTTCATCAAGCTCTGCAAGAAACAGTATTATGATGGTACCATCTTTCACAGGTCCATCAGGAA
  CTTTGTGATCCAGGGCGGTGACCCCACAGGTACAGGCACAGGTGGAGAGTCATTCTGGGGCAAGCCTTTC
  AAAGATGAGTTCCGTCCCAACCTTTCACACACGGGCCGTGGGGTGCTCAGCATGGCCAATTCGGGGCCCA
  ACACCAACAAATCTCAGTTCTTCATCACATTCCGATCCTGCGCTTACCTGGATAAGAAGCATACCATCTT
  TGGACGGGTTGTTGGGGGCTTTGACACGCTGACAGCCATGGAGAATGTGGAGAGTGACCCTAAAACTGAC
  CGTCCTAAGGAGGAAGTGCTCATATGTACAACCACAGTGTTTGTGGACCCCTATGAGGAGGCTGATGCCC
  AGATTGCCCAGGAACGGAAGAAGACACAGCATCAGGTGGATCCAGAGGCCAAGGTCAAGATGAGTCAGCC
  CCAGCCTGGAAACCAGGGACCCCAGACATACCGCCAGGGGGTGGGCAAGTACATCCACCCTGCGGCCACG
  AAACGATCAGCAGAGGAAGAACCATCGACCAGCACTGCCACCCCCACGGCCAAGAAAAGGCCCAGTCGGG
  GCTTTGGGGACTTCAGCTCCTGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252445
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252445.1, NP_001239374.1
• RefSeq Size: 1812 bp
• RefSeq ORF: 1566 bp
• Locus ID: 66053
• UniProt ID: Q9D787
• Cytogenetics: 16 A3
• Gene Summary: Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation. May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.