Ralgps1 (NM_001290572) Mouse Untagged Clone

CAT#: MC228127

Ralgps1 (untagged) - Mouse Ral GEF with PH domain and SH3 binding motif 1 (Ralgps1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ralgps1
• Synonyms: 5830418G11Rik; AI853783; AI854138; mKIAA0351; RALGEF2; RalGEF 2; RALGPS1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228127 representing NM_001290572
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAAGAGGAATGGTCTGATGGCTAGCGTGTTGGTCACCTCTGCCACTCCACAGGGCAGCAGCAGCT
  CAGACTCGCTGGAGGGCCAGAGCTGCGACTATGCCAGCAAGAGCTACGATGCTGTCGTCTTCGACGTTCT
  GAAAGTGACTCCCGAGGAGTTTGCCAGTCAGATCACATTGATGGACATCCCCGTGTTCAAAGCCATCCAG
  CCAGAGGAGTTGGCCAGTTGTGGATGGAGTAAAAAGGAGAAACACAGTCTTGCCCCCAACGTCGTGGCCT
  TTACCCGGAGGTTCAACCAGGTCAGTTTTTGGGTCGTACGAGAAATTCTAACAGCACAGACTTTAAAAAT
  AAGGGCAGAAATCCTGAGCCATTTTGTGAAAATAGCCAAGAAACTTCTAGAACTGAACAACCTTCATTCT
  CTCATGTCTGTGGTGTCAGCATTACAAAGTGCTCCCATCTTCAGGCTGACAAAAACCTGGGCTCTTTTGA
  ATCGAAAAGACAAGACTACTTTTGAGAAACTTGACTACCTGATGTCCAAAGAAGACAACTACAAGCGAAC
  CCGGGACTACATCCGAAGTCTGAAGATGGTGCCCAGCATTCCCTATCTAGGAATCTACCTTCTGGACTTG
  ATCTACATTGATTCTGCATACCCTGCCTCTGGCAGCATCATGGAAAACGAACAACGATCCAACCAGATGA
  ACAACATCCTCCGAATCATTGCCGATTTACAAGTTTCCTGTAGCTATGATCACCTCACTACCCTGCCACA
  TGTGCAAAAGTACCTGAAGTCTGTGCGCTACATCGAGGAGCTCCAGAAGTTTGTAGAAGATGATAATTAC
  AAGTTGTCACTCAGAATCGAACCAGGAAGCAGCTCTCCAAGACTAGTTTCGTCCAAGGAAGATCTTGCAG
  GCCCCTCTGCCGGCTCCAGCTCAGCCAGGTTCCGCCGGAGGCCCACCTGTCCAGATACATCTGTGGCCGG
  CAGCCTCCCCACACCGCCAGTCCCCAGACACAGGAAGAGCCACAGCCTAGGCAACAACAGGGGACGTCTT
  TATGCCACGCTGGGACCTAACTGGCGGGTTCCAGTTCGGAATTCTCCCAGAACTCGGAGCTGTGTCTACA
  GCCCCACGAGCCCTTGCACCTGTACTGTGGGAAGCTCAGCCACTGTGCCCACTATGGAGGGGCCTCTGAG
  ACGGAAAACCCTGCTCAAGGAAGGCCGGAAGCCTGCACTGTCCTCGTGGACCAGGTACTGGGTTGTACTC
  TCAGGAGCCACCCTCCTTTACTATGGAGCCAAGTCTTTGCGGGGTACAGACAGAAAACATTATAAATCTA
  CACCTGGCAAGAAGGTCTCCATCGTAGGCTGGATGGTGCAGCTGCCAGATGACCCCGAGCACCCAGATAT
  CTTCCAGCTGAATAACCCTGACAAAGGCAATGTTTACAAGTTCCAGACGGGCTCCCGTTTCCATGCGATA
  CTGTGGCACAAGCATTTGGATGATGCATGTAAAAGCAGCAGGCCTCAGGTACCTGCAAACCTTATGTCCT
  TTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290572
• Insert Size: 1548 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290572.1, NP_001277501.1
• RefSeq Size: 6131 bp
• RefSeq ORF: 1548 bp
• Locus ID: 241308
• UniProt ID: A2AR50
• Cytogenetics: 2 B
• Gene Summary: Guanine nucleotide exchange factor for the small GTPase RALA. May be involved in cytoskeleton organization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two exons that result in the loss of an in-frame segment in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a.