Gba (NM_001077411) Mouse Untagged Clone

CAT#: MC228122

Gba (untagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gba
• Synonyms: betaGC; GBA1; GC; GCase; GLUC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228122 representing NM_001077411
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCAGGCTCATCGGATTCTTCCTATTTCAGGCGGTATCTTGGGCATATGGTGCCCAACCCTGCA
  TCCCCAAAAGCTTTGGCTACAGCTCAGTGGTCTGTGTCTGTAATGCATCGTACTGTGACTCTCTTGACCC
  CGTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTACGAGAGCACTCGACGTGGACGTCGGATGGAGCTG
  AGTGTCGGGGCCATCCAGGCCAATCGCACTGGCACAGGGTTACTACTCACTTTGCAGCCAGAAAAGAAGT
  TCCAGAAAGTGAAAGGATTTGGAGGCGCCATGACAGACGCCACTGCGCTCAACATCCTTGCTTTGTCCCC
  ACCTACTCAGAAGCTGCTACTCAGATCCTACTTCTCTACCAACGGAATTGAATATAACATCATCCGGGTA
  CCCATGGCCAGTTGTGACTTCTCCATCCGTGTCTATACCTATGCTGACACCCCTAACGACTTCCAGTTAT
  CCAACTTCAGCCTCCCAGAAGAAGACACCAAGCTCAAGATACCCCTGATTCACCAAGCCCTGAAGATGTC
  CTCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAAGAGTG
  AATGGGAAGGGGTCGCTCAAGGGTCAGCCAGGGGATATCTTTCACCAGACCTGGGCCAATTACTTTGTCA
  AGTTTCTGGATGCTTATGCTAAGTATGGCCTAAGATTCTGGGCAGTGACAGCGGAGAATGAACCTACAGC
  AGGGCTCTTCACGGGGTACCCCTTCCAGTGCCTGGGCTTCACTCCTGAACACCAGAGAGACTTCATTTCC
  CGTGACCTAGGGCCAGCCCTTGCCAACAGTTCCCATGATGTGAAGCTACTCATGCTAGATGACCAACGCT
  TGCTGCTACCCCGCTGGGCAGAGGTGGTGCTCTCTGATCCAGAGGCAGCTAAGTACGTTCATGGCATTGC
  TGTTCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC
  AACACAATGCTCTTTGCTTCGGAGGCCTGCGTGGGCTCCAAGTTCTGGGAACAGAGTGTTCGGTTGGGCT
  CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC
  GGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTTGTCGATAGCCCCATT
  ATTGTCGACATCCCCAAAGACGCATTTTACAAACAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT
  TCATTCCCGAGGGATCCCAGAGAGTGGCGTTGGTGGCCAGTGAGAGCACTGACTTGGAAACAGTAGCACT
  GTTACGCCCTGACGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCTTCGGAGGATGTCCCTCTTACCATC
  AGTGATCCTGACCTGGGCTTCCTGGAGACCGTGTCACCTGGCTACTCCATTCACACTTACCTGTGGCGTC
  GCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077411
• Insert Size: 1548 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077411.2, NP_001070879.1
• RefSeq Size: 2069 bp
• RefSeq ORF: 1548 bp
• Locus ID: 14466
• UniProt ID: P17439
• Cytogenetics: 3 39.01 cM
• Gene Summary: Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (By similarity). Also plays a role in cholesterol metabolism (PubMed:24211208). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (By similarity). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the shortest transcript and encodes the functional protein. Variants 1 and 2 encode the same protein.