Etv3 (NM_001083318) Mouse Untagged Clone

CAT#: MC228118

Etv3 (untagged) - Mouse ets variant 3 (Etv3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Etv3
• Synonyms: AI414410; METS; Pe1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228118 representing NM_001083318
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCAGGCTGTAGCATCGTGGAAAAGCCAGAAGGAGGTGGAGGGTATCAGTTTCCGGATTGGGCCT
  ACAAAGCCGAGTCGTCGCCGGGCTCCCGGCAGATCCAGCTGTGGCACTTCATCCTGGAACTGCTGCAGAA
  GGAAGAGTTCCGCCATGTCATCGCCTGGCAGCAGGGAGAGTACGGGGAGTTTGTCATCAAGGATCCAGAT
  GAAGTGGCTCGCCTCTGGGGCCGCAGGAAGTGCAAACCACAGATGAACTATGACAAGCTGAGCCGGGCCC
  TCAGATACTATTACAACAAGAGGATCCTTCATAAAACAAAAGGGAAAAGGTTTACTTACAAGTTTAACTT
  CAACAAGCTCGTGATGCCCAACTACCCGTTCATCAACATTCGCTCGAGTGGTGTGGTTCCTCAGAGTGCG
  CCACCAGTGCCAACAGCCTCCTCCCGGTTCCATTTCCCACCTCTAGACAGCCATTCTCCTACTGGCGATG
  TGCAACCGGGTCGCTTCTCTGCCAGCTCACTGAGTGCTTCTGGCCCTGAGTCAGGGGTTACCACTGACAG
  GAAGGTCGAGCCTTCGGACCTGGAAGATGGCTCGGCCTCTGACTGGCACCGGGGCATGGACTTCATGCCC
  TCCCGAAATGCTCTGGGCGGAGGAGCAGTCGGCCACCAGAAACGCAAGCCTGACATACTGCTTCCTCTCT
  TCACCCGGCCAGCCATGTACCCTGACCCGCACAGTCCCTTCGCTATCTCTCCGGTCCCTGGCCGCGGAGG
  GGTCCTTAATGTCCCCATCTCACCAGCCCTGTCCCTGACTCCCACCATGTTCTCCTACAGTCCCTCACCA
  GGCCTGAGCCCCTTCACCAGCAGCAGTTGCTTCTCCTTCAACCCAGAGGAAATGAAACACTACCTTCATT
  CTCAAGCCTGTTCGGTGTTCAACTACCATCTGAGTCCCCGGACTTTCCCCCGTTACCCAGGGCTCATGGT
  CCCACCGCTGCAGTGCCAAATGCATCCTGAGGAGCCTTCCCAGTTCTCCATCAAGCTGCAGCCCCCGCCG
  GCTGGACGGAAGAACCGCGAGAGGGTAGAGAGCCGGGAGGAGGCTGTTCGCGGCTCTGTGCCCGCCAGCG
  CTCCTGTTCCCTCTCGGATTAAGGTAGAGCCGGCCACAGAGAAGGATCCTGACAGCCTCCGGCAGTCAAC
  CCAGGGAAAGGAGGAACAGACCCAAGAAGTGGACAGCATGCGGAGCAGGACCATAGAAGAGGGGAAAGGC
  ACCGGGTTTGCCCACCCCTCACCCACCTGGCCCTCTGTGTCCATTAGCACTCCCAGTGACGAACCCCTAG
  AGGGGACCGAAGACAGTGAGGACAGGTCTGTCAGGGAGCCCGGTGTACCCGAGAAGAAAGAAGACGCCCT
  GATGCCCCCTAAGCTTCGGCTGAAGAGGCGGTGGAATGACGACCCTGAAGCCAGGGAGCTCAACAAAACG
  GGCAAGTTCCTCTGGAATGGGGCAGGACCCCAGGGCCTGGCCACAACGGCCACTGCTGCCGCTGATGCTT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083318
• Insert Size: 1542 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083318.2, NP_001076787.1
• RefSeq Size: 5143 bp
• RefSeq ORF: 1542 bp
• Locus ID: 27049
• UniProt ID: Q8R4Z4
• Cytogenetics: 3 F1
• Gene Summary: Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.[UniProtKB/Swiss-Prot Function]