Rnpc3 (NM_001287015) Mouse Untagged Clone

CAT#: MC228115

Rnpc3 (untagged) - Mouse RNA-binding region (RNP1, RRM) containing 3 (Rnpc3), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnpc3
• Synonyms: 2810441O16Rik; AI447568; C030014B17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228115 representing NM_001287015
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGCCTGAGCCGCCGATGCCGCTGTCCCGAGGCGGCCCAGGCTCCGCTTCGCTTTCTCCGCCCC
  GGGGAGACCGCACCCTCCTCGTCAGGCACCTGCCGGCCGAGCTGACGGCTGAGGAGAAGGAGGACTTACT
  GAAGTACTTCGGGGCGCAGTCGGTGCGCGTCCTGTCAGATAAGGGGCGCCTGAAACACACAGCATTTGCT
  ACTTTCCCTAATGAAAAAGCAGCAATAAAGGCACTGACGAGACTCCATCAGCTGAAACTTCTAGGTCATA
  CTTTAGTTGTTGAATTTGCAAAAGAGCAAGATCGAGTTCACTCCCCATGTTCCACCTCAAATACAGAGAA
  AAAGAAAAGGTTGGATGACACTGTGGAAAATGATAAAGAGAAGAAAGAACCTGACATTTTAACAGTAGAA
  AATGGAATTGCACCAAATCATGGGCTGACTTTTCCTCTGAATTCATGCCTTAAATATATGTATCCTCCAC
  CATCAAGCACAATCCTCGCAAATATAGTAAATGCTCTGGCAAGTGTCCCAAAATTCTATGTACAGGTGCT
  TCATCTTATGAATAAAATGAATTTGCCCACACCATTTGGACCGATTACTGCACGACCTCCTATGTATGAG
  GACTATATGCCATTGCATGCACCCCTTCCACCTACATCCCCTCAACCGCCTGAGGAGCCTCCCTTACCAG
  ATGAGGATGAGGATTTATCTAGCAAAGAATCAGAATATGAAAGCAGTGATGAGGAGGACCGACAGAGAAT
  GAACAGATTAATGGAACTAGCAAATCTTCAGCCCAAAAGACCCAAAACAGAAAAGCCACGCCACGTGAGA
  AAAAAGCGAAAAATAAAGGATATGTTGAATATACCTTCATCAGCTTCACATAGTTTGCATCCTGTGCTGT
  TACCTTCGGATGTATTTGACCAGCCACAATCTGTAGGTAATAAAAAAATTGAGTTCAATATATCAACCAA
  CATGCCTGCAGCATTTAACAAAGATTTGGAGACACAACCAAATAATGAAGAAGAAAATTCTGATTCACCA
  GACACTGGACTTGATTCAAATACAGGATTTGGCAAAATCTTCCCTAAACCTAATTTGAACATCACAGAAG
  AGATTACAGAAGACTCTGATGAAATACCTTCACAATTTATTTCTAGAAAAGAGTTGGAAAAGGGTCGTAT
  TTCTAGAGAAGAAATGGAAACACTTTCAGTTTTTAGAAGTTATGAACCTGGTGAACCAAACTGCAGAATT
  TATGTAAAGAATTTAGCTAGACATGTTCAGGAAAAGGACCTTAAATTTATATTTGGAAGATACGTTGACT
  TTTCTTCAGAAACACAGCGGATCATGTTTGATATTCGCTTAATGAAAGAAGGACGCATGAAAGGACAAGC
  TTTTGTTGGACTTCCAAATGAAAAAGCAGCCGCTAAAGCGTTGAAAGAAGCTAATGGCTATGTTCTTTTT
  GGAAAACCCATGGTGGTTTTTGCTCGATCTGCTAGACCAAAACAGGATTCTAAAGAAGGAAAACGGAAGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287015
• Insert Size: 1542 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287015.1, NP_001273944.1
• RefSeq Size: 3885 bp
• RefSeq ORF: 1542 bp
• Locus ID: 67225
• UniProt ID: Q3UZ01
• Cytogenetics: 3 F3
• Gene Summary: Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform b which is shorter than isoform a.