Ppp3ca (NM_001293622) Mouse Untagged Clone

CAT#: MC228107

Ppp3ca (untagged) - Mouse protein phosphatase 3, catalytic subunit, alpha isoform (Ppp3ca), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp3ca
• Synonyms: 2900074D19Rik; Caln; Calna; CN; CnA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228107 representing NM_001293622
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGCCCAAGGCGATTGATCCCAAGTTGTCGACGACCGACAGGGTGGTGAAAGCCGTTCCATTTC
  CACCAAGTCACCGGCTGACAGCAAAGGAAGTGTTTGATAATGATGGGAAACCTCGTGTGGATATCTTAAA
  AGCACATCTCATGAAGGAGGGCAGGCTGGAAGAAAGTGTTGCATTGAGAATAATAACAGAGGGTGCTTCG
  ATTCTCCGACAGGAAAAAAACTTGCTGGATATCGACGCACCAGTCACAGTTTGTGGGGACATCCATGGAC
  AATTCTTTGACTTGATGAAGCTCTTTGAAGTGGGAGGATCTCCTGCCAACACTCGCTACCTCTTCTTAGG
  GGACTATGTTGACAGAGGGTACTTCAGTATCGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTTTAC
  CCCAAAACACTGTTTTTACTTCGCGGAAACCATGAATGTAGGCACCTCACAGAGTATTTCACGTTTAAAC
  AAGAATGTAAAATAAAGTATTCAGAACGCGTTTATGACGCCTGTATGGATGCCTTCGACTGCCTTCCCCT
  GGCTGCGCTAATGAACCAGCAGTTCCTGTGTGTACACGGTGGTTTGTCTCCAGAGATTAACACTCTAGAT
  GACATCAGAAAATTAGACCGATTCAAAGAACCACCTGCTTATGGGCCCATGTGTGACATCCTATGGTCAG
  ACCCCCTGGAGGACTTTGGAAATGAGAAGACTCAGGAACATTTCACTCACAACACAGTCAGAGGCTGTTC
  GTACTTCTACAGTTACCCAGCTGTGTGTGACTTCCTGCAGCACAATAATTTGTTGTCCATACTCCGCGCC
  CACGAAGCCCAGGATGCAGGGTACCGCATGTACAGGAAAAGCCAAACAACAGGCTTCCCGTCTCTAATTA
  CAATCTTCTCGGCACCAAATTACTTAGATGTGTACAATAACAAAGCTGCAGTGTTGAAGTACGAGAACAA
  TGTGATGAACATCAGGCAGTTCAACTGCTCCCCGCATCCGTACTGGCTCCCAAATTTCATGGATGTTTTC
  ACCTGGTCGCTGCCATTTGTTGGGGAGAAAGTGACTGAGATGCTGGTCAATGTTCTCAACATCTGCTCCG
  ACGATGAACTGGGGTCAGAAGAAGATGGATTTGACGGAGCCACGGCCGCAGCCCGGAAGGAAGTCATCAG
  AAACAAGATCCGAGCAATAGGCAAAATGGCCAGAGTGTTCTCAGTTCTCAGAGAAGAGAGTGAGAGTGTC
  CTGACACTGAAGGGCCTGACCCCAACTGGCATGCTCCCCAGCGGAGTGCTCTCTGGCGGGAAACAGACTC
  TGCAAAGCGCCATCAAAGGATTTTCACCACAACATAAGATCACTAGCTTCGAGGAGGCCAAGGGCTTAGA
  CCGAATTAACGAGAGGATGCCACCTCGCAGAGACGCCATGCCCTCTGACGCCAACCTTAACTCCATCAAC
  AAGGCTCTCGCCTCAGAGACTAACGGCACGGACAGCAATGGCAGTAATAGCAGCAATATCCAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001293622
• Insert Size: 1536 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293622.1, NP_001280551.1
• RefSeq Size: 4832 bp
• RefSeq ORF: 1536 bp
• Locus ID: 19055
• UniProt ID: P63328
• Cytogenetics: 3 G3
• Gene Summary: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:7791792, PubMed:26794871). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (By similarity). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (By similarity). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (By similarity). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (By similarity). Dephosphorylates and inactivates transcription factor ELK1 (By similarity). Dephosphorylates DARPP32 (By similarity). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.