Slc7a7 (NM_001253679) Mouse Untagged Clone

CAT#: MC228103

Slc7a7 (untagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 7 (Slc7a7), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc7a7
• Synonyms: AI790233; my+lat1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228103 representing NM_001253679
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCAACAGCACCAAGTATGAAGTGGCTGCTCAGCACGAGGCCGACGATGGCTCTGCTCTCGGGGATG
  GGGCCAGCCCAGTGGCGGAGCAGGTCAAACTGAAGAAGGAGATCTCCCTGCTTAATGGCGTGTGTCTCAT
  AGTGGGGAACATGATCGGCTCCGGCATCTTTGTCTCCCCTAAGGGTGTGCTCATGTACAGTGCCTCTTTT
  GGCCTCTCACTGGTCATCTGGGCTGTCGGGGGCATTTTCTCCGTCTTTGGGGCCCTTTGTTACGCTGAAC
  TGGGTACCACCATTAAGAAATCCGGGGCCAGCTATGCTTATATCCTGGAGGCCTTCGGGGGATTCCTTGC
  GTTCATCCGCCTCTGGACTTCTCTGCTCATCATTGAACCCACCAGCCAGGCCGTCATTGCCATCACCTTT
  GCCAACTACATGGTGCAGCCCCTCTTCCCGAGCTGTGGCGCTCCCTATGCCGCCGGCCGCCTGCTGGCCG
  CTGCCTGCATCTGTCTCTTAACCTTCATTAACTGTGCCTATGTCAAGTGGGGAACCCTGGTCCAAGATAT
  TTTCACCTATGCTAAGGTGTTGGCGCTGATTGCAGTCATCATTGCAGGCATTGTTAGACTTGGCCAGGGA
  GCCACAGCTAACTTTGAGAACTCCTTTGAGGGCTCATCCTTTGCAATGGGTGACATTGCTCTGGCACTCT
  ACTCAGCCCTGTTCTCCTACTCGGGCTGGGACACCCTTAACTATGTCACCGAAGAGATCAGGAACCCCGA
  GAGGAACTTGCCCCTCTCCATTGGCATTTCCATGCCAATCGTCACCATCATCTACCTCTTGACCAATGTG
  GCCTATTACAGTGTGCTAGACATAAAGGAAATCCTGGCCAGTGACGCTGTTGCCGTGACCTTTGCAGATC
  AAATTTTTGGAGTATTCAATTGGATAATTCCAGTAGCGGTTGCATTCTCTTGCTTTGGTGGGCTCAACGC
  CTCCATCGTGGCTGCTTCCAGGCTTTTATTTGTGGGCTCGAGAGAAGGCCACCTCCCTGACGCCATCTGT
  ATGGTTCATGTTGAACGGTTCACACCAGTGCCTTCTCTACTCTTCAATGGTGTGTTGTCCCTGGTCTACC
  TGTGCGTGGAGGACATCTTCCAGCTCATTAACTACTATAGCTTCAGCTACTGGTTCTTTGTGGGCCTTTC
  TATTGTGGGTCAGCTTTATCTACGCTGGAAGGACCCCGACCGGCCTCGCCCTCTCAAGCTCAGCCTTTTC
  TTCCCCATCATCTTCTGCCTCTGCACCATCTTCCTGGTGGCTGTTCCACTTTACAGCGACACCATCAACT
  CCCTCATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTTCTTCATCATCAGAGTGCCAGAGCA
  CAAACGACCACTGTTCCTCCGCAGGATTGTAGCCTCTATCACACGGTACCTCCAGATCCTCTGTATGTCA
  GTTGCTGCAGAAATGGACTTGGAAGATGGAGAGTTGTCCAAACAAGATCCCAAGTCTAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253679
• Insert Size: 1533 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253679.1, NP_001240608.1
• RefSeq Size: 2126 bp
• RefSeq ORF: 1533 bp
• Locus ID: 20540
• UniProt ID: Q9Z1K8
• Cytogenetics: 14 C2
• Gene Summary: Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis via transport of L-arginine, and is involved in the transport of L-arginine in monocytes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.