Mier1 (NM_001286223) Mouse Untagged Clone

CAT#: MC228102

Mier1 (untagged) - Mouse mesoderm induction early response 1 homolog (Xenopus laevis (Mier1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mier1
• Synonyms: 4933425I22Rik; 5830411K19Rik; er1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228102 representing NM_001286223
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCATCTGTTGAGTCTTCAAGTCCAGGAGGCTCAGCAACATCAGAGGACCATGAGTTTGATCCAT
  CAGCTGACATGCTGGTTCATGATTTTGATGATGAGCGAACATTAGAAGAAGAAGAAATGATGGAAGGAGA
  GACAAACTTCAGTTCTGAAATAGAGGATCTTGCGAGGGAAGGCGACATGCCAATCCACGAGCTGCTCAGC
  CTCTATGGTTATGACAGCACTGTTCGGTTACCTGAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAAGGTG
  AAGATGATGAAGATGCTGATAATGATGATAACAGTGGCTGTAGTGGAGAAAATAAAGAAGAGAATATAAA
  GGATTCATCGGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCCTCACAGTCTGTTACTTCCCAA
  GATGCTCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAATAGAAGAAGAAT
  CTGAAGAAGATGAAGATTATATTCCCTCAGAAGACTGGAAAAAGGAAATCATGGTGGGCTCCATGTTTCA
  AGCTGAGATTCCAGTTGGTGTTTGTAGATACAAAGAAAATGAAAAAGTGTATGAAAATGATGATCAGCTT
  CTGTGGGATCCAGAGTGTTTACCAGAAGAGAAAGTGGTTGTCTTTCTTAAGGATGCGTCTAGAAGGACAG
  GGGATGAGAAAGGTGTGGAAGCAATCCCTGAAGGCTCGCATATCAAAGACAATGAGCAGGCTTTATATGA
  GTTGGTTAAATGCAGCTTTGATACGGAAGAAGCCTTGAGAAGACTGAGATTTAATGTCAAAGCAGCTCGA
  GAGGAGTTATCTGTGTGGACAGAGGAAGAGTGTAGAAATTTTGAGCAAGGGCTGAAGGCCTATGGAAAAG
  ATTTTCATCTGATTCAGGCTAATAAAGTCCGAACAAGATCAGTTGGTGAATGTGTAGCATTCTATTACAT
  GTGGAAAAAGTCTGAGCGCTATGATTTCTTTGCTCAGCAAACAAGGTTTGGAAAAAAGAAATATAATCTT
  CATCCTGGTGTAACGGATTACATGGATCGTCTTTTGGATGAAAGTGAAAGTGCTGCTTCTAGCCGAGCAC
  CATCCCCCCCTCCCACTGCCTCAAATAGCAGTAACAGCCAGTCCGAGAAAGAAGATGGCGCTGTCAGCAG
  TAGGAATCAGAACGGTGTCTCATCTAATGGACCAGGAGAAATACTAAACAAAGAAGAAGTAAAAGTTGAA
  GGGTTACATGTTAATGGACCAACAGGTGGAAATAAGAAACCACTTCTTACAGATATGGACACTAATGGTT
  ATGAAGCAAATAACCTGACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGA
  TGAAAAAAATGAGAGACCTGCCAAAAGGCGGCGGATAAACAGCAGTGGGAAAGAAAGTCCGGGCTCATCT
  GAGTTTTTCCAGGAAGCAGTCTCACATGGGAAGTTTGAGGAACATGAAAACACGAATGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286223
• Insert Size: 1533 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286223.1, NP_001273152.1
• RefSeq Size: 4631 bp
• RefSeq ORF: 1533 bp
• Locus ID: 71148
• UniProt ID: Q5UAK0
• Cytogenetics: 4 C6
• Gene Summary: Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks an alternate exon in the 5' coding region and initiates translation at an alternate start codon, compared to variant 4. It encodes isoform d, which is shorter and has a distinct N-terminus, compared to isoform c.