Ate1 (NM_001271343) Mouse Untagged Clone

CAT#: MC228099

Ate1 (untagged) - Mouse arginyltransferase 1 (Ate1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ate1
• Synonyms: AI225793; AW547406
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228099 representing NM_001271343
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGTGGTGGAATACAAGGGCCTGAAGGCCGGCTACTACTGCGGCTACTGCGAGTCCAGGGAGG
  GCAAGACGTCGTGTGGCATGTGGGCACATTCCATGACAGTGCAGGATTATCAGGATCTTATAGACCGAGG
  ATGGAGAAGAAGTGGGAAATATGTGTACAAACCTGTCATGGATCAAACATGCTGTCCTCAGTATACAATA
  AGGTGTCATCCTTTACAGTTTCAGCCATCAAAATCTCACAAGAAAGTTTTGAAAAAAATGCTGAAATTTC
  TGGCTAAAGGAGAGATCTCGAAAGGCAATTGTGAGGATGAGCCCATGGATTCTACAGTGGAGGATGCTGT
  TGACGGTGACTTTGCATTAATTAACAAGCTGGATATAAAGTGTGATCTCAAAACACTCAGTGACCTCAAA
  GGAAGCATAGAGAGTGAAGAGAAGGAGAAAGAAAAGAGTATAAAGAAAGAAGGGTCTAAAGAATTCATTC
  ATCCACAATCTATAGAGGAGAAGTTGGGCTCTGGTGAACCATCACATCCAATCAAAGTTCATATTGGTCC
  TAAGCCAGGCAAAGGGGCTGACTTGAGTAAGCCTCCATGTCGGAAAGCAAGGGAAATGAGGAAAGAAAGG
  CAAAGATTAAAACGGATGCAGCAGGCCTCAGCTGCAGCCTCGGAGGCTCAAGGTCAGCCAGTCTGTTTGT
  TACCAAAGGCTAAATCCAACCAGCCCAAGTCACTGGAAGATTTGATTTTTCAATCTTTACCAGAAAATGC
  ATCGCACAAGTTAGAGGTGAGGCTAGTACCTGCCTCCTTTGAGGACCCAGAGTTCAACTCATCCTTCAAC
  CAGTCCTTTTCTTTATATACCAAGTATCAAGTGGCTATACACCAGGAAGCACCTGAAATATGTGAAAAGT
  CTGAGTTTACAAGATTCCTTTGCAGCTCACCATTGGAGGCAGAGCACCCTGCTGATGGACCAGAATGTGG
  TTATGGCTCCTTTCACCAGCAGTACTGGCTCGATGGGAAGATCATTGCTGTGGGGGTGTTAGACATTCTC
  CCGTACTGTGTGTCTTCTGTGTATCTCTACTACGATCCTGATTATTCATTTCTGTCTTTGGGTGTCTATT
  CAGCATTAAGAGAAATTGCTTTTACTAGACAACTGCATGAGAAAACATCGCAACTCAGCTATTATTATAT
  GGGTTTCTACATTCATTCCTGTCCCAAGATGAGATACAAGGGTCAATATAGACCTTCTGATTTGCTGTGT
  CCTGAGACGTATGTCTGGGTGCCCATTGAGCAGTGCCTGCCTTCTCTGGACAACTCCAAGTACTGCCGTT
  TCAACCAGGACCCCGAAGCAGAGGATGAAGGACGCAGTAAGGAACTTGACCGACTAAGGGTGTTTCACAG
  ACGATCTGCCATGCCTTACGGTGTTTATAAGAATCACCAAGAAGACCCAAGTGAGGAGGCTGGTGTGCTG
  GAGTATGCAAACCTCGTAGGACAGAAGTGCTCGGAGAGGATGCTGCTGTTCAGACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271343
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271343.1, NP_001258272.1
• RefSeq Size: 5020 bp
• RefSeq ORF: 1530 bp
• Locus ID: 11907
• Cytogenetics: 7 F3
• Gene Summary: Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 5' structure and an alternate in-frame exon in the coding region, compared to variant 1. These differences cause translation initiation at an upstream AUG and result in an isoform (2) that has a distinct N-terminus and is shorter, compared to isoform 1.