Banp (NM_001285981) Mouse Untagged Clone

CAT#: MC228097

Banp (untagged) - Mouse BTG3 associated nuclear protein (Banp), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Banp
• Synonyms: AA408158; SMAR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228097 representing NM_001285981
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCAGAGCAGGACCTGGCGGATGTGGTTCAGATTGCAGTGGAAGACCTGAGCCCTGATCACCCAG
  TTGTTTTGGAGAATCATGTCGTGACAGATGATGATGAACCTGCCTTGAAGCGCCAGCGACTAGAGATCAA
  TTGCCAGGACCCCTCTATAAAGTCTTTCCTGTACTCTATTAACCAGACGATATGTTTGCGGTTGGATAGC
  ATTGAGGCCAAGCTGCAAGCTCTCGAGGCCACTTGCAAATCTCTGGAAGAGAAGCTAGACCTGGTCACCA
  ATAAACAGCACAGTCCCATCCAGGTCCCCATGGTGGCAGGTTCCCCACTTGGCGCCACCCAGACCTGCAA
  CAAAGTGCGATGCGCTGTTCCTGGGCGTCGGCAGAACACCATCGTGGTGAAAGTGCCTGGTCAGGACGAC
  AGCCACAACGAAGATGGGGAGAGCGGGTCAGAGGCCAGTGACTCCGTGTCTAACTGTGGCCAGCCAGGAA
  GCCAGAACATTGGAAGCAACGTCACACTCATCACCCTGAACTCCGAAGAGGACTATCCCAATGGCACCTG
  GCTGGGCGATGAGAATAACCCTGAGATGCGGGTACGCTGTGCCATCATCCCTTCCGACATGTTGCACATC
  AGCACCAACTGTCGCACGGCCGAGAAGATGGCGCTGACACTGCTGGACTACCTGTTCCACCGTGAGGTGC
  AGGCTGTGTCCAACTTGTCCGGCCAGGGCAAGCACGGGAAGAAGCAGCTGGACCCCCTCACCATCTACGG
  CATCCGGTGTCACCTCTTCTATAAATTTGGAATCACGGAATCTGACTGGTATCGGATCAAGCAGAGCATT
  GACTCCAAGTGCCGGACAGCCTGGCGGCGGAAGCAGCGAGGCCAGAGCCTGGCGGTCAAGAGCTTCTCTC
  GGAGGACGCCATCCTCATCCTCTTACAGTGCCTCAGAGACCATGATGGGAACCCCTCCTCCCACCAGTGA
  GCTACAGCAGTCACAGCCACAGGCCCTACACTACGCCCTGGCCAACGCCCAGCAGGTCCAGATCCACCAG
  ATTGGGGAGGATGGACAGGTGCAAGTAATCCCACAGGGCCACCTCCACATTGCCCAGGTGCCTCAAGGGG
  AGCAGGTGCAGATCACACAGGACAGCGAGGGCAATCTGCAGATCCATCATGTGGGTCAGGATGGCCAGTC
  GTGGGGCCTGTGCCAGAATCCCATTCCTGTCAGCGGTGACTCAGTGGCCCAGGCTAATCCCTCCCAGCTT
  TGGCCTCTGGGAGGAGACACACTTGATCTGCCTGCTGGAAATGAAATGATCCAGGTACTGCAGGGTGCTC
  AGCTCATAGCCGTGGCCTCTTCAGACCCTGCTGCTACAGGAGTAGATGGGTCGCCTCTCCAGGGCAGTGA
  CATTCAGGTTCAGTATGTCCAGCTGGCGCCTGTGAGTGACCACACAGCCGCAGCGCAGACCGCAGAGGCC
  CTGCAGCCCACTCTGCAGCCCGACATGCAGCTTGAACATGGGGCCATCCAGATCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285981
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285981.1, NP_001272910.1
• RefSeq Size: 5381 bp
• RefSeq ORF: 1530 bp
• Locus ID: 53325
• UniProt ID: Q8VBU8
• Cytogenetics: 8 70.82 cM
• Gene Summary: Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest and inhibits tumor growth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region compared to variant 2. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.