Tcp1 (NM_001290712) Mouse Untagged Clone

CAT#: MC228088

Tcp1 (untagged) - Mouse t-complex protein 1 (Tcp1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcp1
• Synonyms: AI528772; c-cpn; CCT; Cct1; Ccta; p63; Tcp-1; Tp63; TRic
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228088 representing NM_001290712
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTAACCATTACTAACGATGGTGCCACCATCCTGAAGTTACTGGAGGTAGAACATCCCGCAGCCA
  AAGTTCTGTGTGAGCTGGCTGACCTGCAAGACAAAGAAGTTGGAGATGGAACTACCTCAGTGGTAATCAT
  TGCAGCGGAGCTTCTGAAAAATGCAGATGAGCTAGTCAAACAGAAAATTCATCCAACATCAGTTATTAGT
  GGCTATCGTCTTGCCTGCAAGGAAGCGGTGCGTTATATCAATGAGAACCTGATTATTAACACAGACGAAC
  TTGGAAGAGACTGTCTGATCAATGCTGCTAAGACATCCATGTCATCCAAAATTATTGGAATAAATGGTGA
  TTACTTTGCTAATATGGTAGTAGATGCTGTGCTTGCTGTTAAATACACAGATGCCAGAGGCCAGCCTCGC
  TATCCAGTCAATTCTGTTAATATTCTGAAAGCCCATGGGAGAAGTCAGATAGAAAGCATGCTGATCAATG
  GCTATGCGCTCAATTGTGTGGTTGGATCTCAGGGCATGCCCAAGAGAATAGTTAATGCAAAAATTGCTTG
  TCTTGACTTCAGCCTGCAGAAAACAAAAATGAAGCTTGGTGTACAGGTGGTTATTACAGACCCTGAGAAA
  TTGGACCAAATTAGACAGAGAGAATCGGATATCACCAAGGAGAGAATTCAGAAGATCCTGGCAACTGGTG
  CCAATGTTATTCTAACCACTGGTGGCATTGATGATATGTATCTCAAGTATTTTGTGGAAGCTGGTGCCAT
  GGCTGTTAGGAGAGTTTTAAAACGAGACCTGAAGCATGTTGCAAAAGCTTCTGGAGCAAGTATCCTGTCT
  ACGCTGGCCAATTTGGAAGGCGAAGAAACTTTTGAAGTGACGATGTTGGGACAAGCGGAAGAGGTCGTAC
  AGGAGAGAATTTGTGATGATGAGCTGATCTTAATCAAAAATACTAAGGCTCGTACATCTGCTTCAATCAT
  CTTACGAGGAGCAAATGATTTCATGTGTGATGAAATGGAGCGCTCTTTACATGATGCTCTTTGTGTGGTG
  AAGAGAGTTTTGGAGTTGAAATCTGTGGTCCCAGGTGGAGGTGCTGTAGAAGCTGCCCTGTCCATATACC
  TGGAAAACTATGCAACAAGCATGGGATCTCGGGAACAGCTTGCTATTGCAGAGTTTGCAAGATCTCTGCT
  TGTGATTCCTAATACACTGGCAGTGAATGCTGCCCAGGACTCCACCGACCTGGTTGCCAAGTTAAGAGCT
  TTTCACAATGAGGCTCAAGTGAACCCGGAACGTAAAAATCTAAAGTGGATTGGTCTTGATTTGGTCCATG
  GGAAACCACGAGACAACAAGCAAGCAGGGGTGTTTGAACCAACCATAGTTAAAGTGAAGAGCCTGAAGTT
  CGCAACAGAGGCTGCAATCACCATCCTTCGGATTGATGATCTGATAAAATTACACCCAGAAAGCAAAGAC
  GATAAACACGGAAGTTATGAAAATGCTGTTCACTCTGGAGCCCTTGATGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290712
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290712.1, NP_001277641.1
• RefSeq Size: 2413 bp
• RefSeq ORF: 1524 bp
• Locus ID: 21454
• UniProt ID: P11983
• Cytogenetics: 17 8.72 cM
• Gene Summary: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1.