Tysnd1 (NM_001272092) Mouse Untagged Clone

CAT#: MC228085

Tysnd1 (untagged) - Mouse trypsin domain containing 1 (Tysnd1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tysnd1
• Synonyms: 1300019N10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228085 representing NM_001272092
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGCAATGGGGACCCTCTATGAGAGTGGCGGAGCAGGCGGGCTGCGTGGTGAGCGCCTCGCGGG
  CCGGACAGCCTGACGCGGGCTCGTGGAGCTGCAGCGGGGTGATTCTGAGCCGTAACCCAGGCCTAGTGCT
  GTGCCACGGAGGCATCTTTACCCCCTTCCTGCGGACGGGCAGCGCGGCGCTGACCCAGACGGGCACCGCC
  TTCCTGCCGGGCGACAGTTGCAGCGATGACCTACGTCTCCACGTGCAGTGGGGACCCACGGCTGCCAGCC
  CTGCCGGTCGCGCGGATCAGGAACTCCCGGGGCTGTGCACGCCTCAGTGCGCAAGCCTGGGCCTCGAACC
  CGGCGCCCCGAGCCGGGCCCGCGCGCGGCCTCTGCAGCCCCCGCGACCAGCGCAGCTGCTGTTGCTGCTC
  AGCTGCCCGGCCTTCCGCTCGCATTTCGCTCGGCTCTTCGGGGCCGATGCGGTCGATCAATGGCACTTCG
  TAAGCTCGGCGCCCGACGATGCGGTTTCGGAGGAGGAAGAGGAGGACCAGCTGCGCGCGCTCGGCTGGTT
  CGCCCTGCTGCGGGTGCAGCGGGGCGCGGCGGCGGAGGAGAGGCGCGGACCGGTGGTGACCGTGGCACCG
  CTCGGGGCCGTGGTCAAGGGCGCACCGCTGTTGGCTTGTGGCTCTCCGTTCGGTGCCTTCTGCCCGGACA
  TCTTCCTCAACACGCTGAGCCGCGGCGTGCTCAGCAACGCGGCCGGGCCGCTGCTGCTCACCGACGCGCG
  CTGCCTGCCGGGCACGGAAGGCGGCGGAGTGTTCGCCGCTCGACCTGCGGGTGCGCTGGTGGCTCTGGTG
  GCCGCGCCGCTCTGCTGGAAGGCCCGCGAGTGGGTGGGCCTCACGCTGCTGTGCGCCGCCGCCCCGCTGC
  TTCAGGTTGCCCGCTGGGCGCTCGCCCGTCTGCACCCCGGCTCCGCTTCCCTGTCTGTGCTGCTGCCGCC
  ACCCGATGTGAGCACGCCACGGGGCCTGCCTCTGCGCGATCTCGGACCCCCGTGGGCGGCCGCGGCGGTG
  CTGGTGGAGTGTGGTACCGTGTGGGGCTCCGGAGTGGTCGTGGCGCCTCGCCTGGTGGTCACCTGTCGCC
  ATGTGGCACCCCGGGAAGCAGCCAGGGTGTTGGTACACTCCGCTACACCCAAGAATGTGGCAATCTGGGG
  ACAAGTGGTGTTTGCCACTCAGGAGACCTCTCCATATGACATAGCAGTGGTGAGCCTGGAAGAGGAGCTG
  AATGGTGTTCCCACGCCTGTCCCTGCTGGGCACTTCCATGAAGGCATTGTCGCCAGCAACACTCGAGACA
  ACAACACGGGAGCCACCTACCCACACCTGAACTTCAGCATCCCCATCACGGTGCTCCAGCCAGCCCTGAA
  GCAGTACAGTCAGACTGGTGACCTGGGTGGCCTCCGAGAGCTGGACCACACCACTGAGCCGGTCAGGGTC
  GTGTGGCGGCTGCAGCGGCCCCTGTCCGAAGTCCCACGGAGCAAGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272092
• Insert Size: 1521 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272092.1, NP_001259021.1
• RefSeq Size: 2100 bp
• RefSeq ORF: 1521 bp
• Locus ID: 71767
• UniProt ID: Q9DBA6
• Cytogenetics: 10 B4
• Gene Summary: This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol, thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter, potentially inactive, peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (4) lacks an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a.