Rrp8 (NM_133951) Mouse Untagged Clone

CAT#: MC228073

Rrp8 (untagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rrp8
• Synonyms: 1500003O22Rik; 2900001K19Rik; AW538116
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228073 representing NM_133951
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCCCGAGTAGCGGAGAGAGGACTTGTAGCCGTCTCACTCGCCAACGCTTCAGCTCACCCCTGA
  TTGAGGTCGTTCTCTGTGTTGTCCAGCCACAAGCGCACCGCCACCGCGTTGCCCTCCCGGCACTGAGTGA
  AAATGTCGTCCATAGCAGCGTCCCGGCACAGAGACCCCGGGACTGGGGAAGCCTCGGGGGACTGAGGGAG
  AACAGAGACAAGAGAATGACTCTCTGTGGACGCAAGCGCCGCCATCTCTTGGCCACATTACGGGCTCTGG
  AAGCAGCATCTCTCTCCCAACAAACCCCCAGCCTACCTGGCAGTGACTCTGAGGAGGAGGAGGAGGTAGG
  AAGGAAGAAGAGACACCTCCAAAGGCCCTCACTTGCCAGCGTCTCAAAGGAAGTAGGGAAGAAAAGAAAA
  GGGAAATGTCAGAAACAGGCGCCATCCATCAGTGACTCTGAGGGGAAAGAAATAAGAAGAAAATGCCACA
  GACAAGCTCCTCCTCTTGGTGGGGTCTCTGCTGGAGAAGAAAAAGGAAAGAGAAAATGCCAGGAATATTC
  CTCTTTACACCTAACCCAGCCCCTGGACAGTGTTGACCAAACAGTTCACAATTCCAGGACGAGTACTGCT
  ACAATTGACCCATCCAAGCCAAGCCCTGAGTCTATGTCACCTAACTCCTCACACACCCTGAGCCGCAAGC
  AGTGGCGGAACCGGCAGAAAAATAAGCGGAGACACAAGAACAAATTTCGGCCACTCCAGACACCAGAGCA
  GGCTCCTCCCAAGGCTTCCATAGAGGAGACTGAGGTGCCTCCTGTCCCAAAGTCAGATAGTCAAGAGTCT
  AGAGCTGGAGCCCTGCGAGCACGCATGACACAACGCCTGGATGGGGCCCGATTTCGCTACCTTAATGAAC
  AGTTGTACTCAGGGCCCAGCAGTGCTGCCCGACGCCTATTCCAGGAAGACCCTGAGGCTTTTCTCCTTTA
  TCACCGTGGCTTCCAGAGACAAGTAAAGAAGTGGCCACTGCACCCAGTGGACCGTATTGCCAAAGATCTC
  CGCCAGAAGCCTGCATCCTTAGTGGTAGCTGACTTTGGCTGTGGAGATTGCCGCCTAGCTTCAAGTGTCC
  GGAACCCTGTGCACTGTTTTGATTTGGCTTCTCTGGACCCCAGGGTCACAGTATGTGACATGGCCCAGGT
  GCCTCTGGAGGATGAATCTGTAGATGTGGCTGTGTTTTGCCTTTCACTGATGGGAACTAACATCAGGGAC
  TTCCTTGAGGAGGCAAATCGAGTGCTAAAAACAGGGGGTCTTCTCAAAGTAGCTGAAGTCAGCAGCCGCT
  TTGAGGATATTCGGACCTTTTTGGGGGCTGTGACCAAACTCGGCTTTAAGATTATCTACAAGGACCTGAC
  CAACAGCCACTTCTTCTTGTTTGACTTTGAAAAGACCGGACCTCCTCGAGTAGGACCCAAAGCCCAACTC
  TCAGGCCTTAAACTTCAGCCCTGTCTCTACAAGCGCAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133951
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133951.1, NP_598712.1
• RefSeq Size: 2890 bp
• RefSeq ORF: 1512 bp
• Locus ID: 101867
• Cytogenetics: 7 E3
• Gene Summary: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.