Mkks (NM_001286981) Mouse Untagged Clone

CAT#: MC228070

Mkks (untagged) - Mouse McKusick-Kaufman syndrome (Mkks), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mkks
• Synonyms: 1300013E18Rik; AI463362; AI957237; Bbs; Bbs6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228070 representing NM_001286981
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGTTAGAAGCTAAGAAGCCATCGTTGTGTAAAACTGAACCATTGACAAGTGAGAAAGTCAGGT
  CCACACTTTCTGTCTTGAAAGGAGTCATAGCCTCATGCTATGGCCCTTCAGGGAGGCTGAAGCAGCTACA
  CAATGGATTGGGAGGTTGTGTGTACACAACCTCACAGTCCTCAGCCCTGCTTCGAAACCTTTCAGTCACC
  CATCCCGTATTAAAGATCCTAACGTCATCCGTGCAGAATCACGTGTCCTGCTTCAGTGACTGCGGCTTAT
  TCACAGCCATTCTGTGCTGCAACTTGATTGAAAATATTCAAAGACTAGATTTGACACCCGCAACTGCTAT
  TAAATTAAATAAATACCTCTTGAGTCTCTGTACCAGTTATCTCAAGTCTGAAGCCTGTAGTTGTCGAATC
  CCAGTTGACTTCAGAAGTACACATACCTTCCTCAGCTTGGTACACAGTATCTTAACAAGCAAACCAGCCT
  GTATGCTCACCAGAAAGGAAACAGATCACATAGGTGCTTTGATTTTGAAAGCTTTTTTACTTACAATTCC
  AGAAAGTACAGAAGAACGAATGGTTTTAGGGAAGAGTATAATTGTTCCCTTAAAGGGCCAAAGAGTTACA
  GATTCTACTGTATTACCTGGACTACTCATTGAAGCATCAGAAGTTCAATTAAGGAGGTTATTACCCACTC
  AAAAGTTCTTCAGTGAGCGCCACGTTATGGCCATCGACAGAGTTGGGGTGACTCTGATGGAATCTCTAAG
  CAAAGTGACAGGAGCAACGCCTATTGGTTCTCTAAACCCAATAGTTTCTACTACTTATGGAAGTGTGAAA
  GATGTGTGCTCTGCAAGATTTGGCTCCAAACATTTTTTTCATCTTCTTCCTAATGAGGCCACTGTCTGCA
  CCTTGCTTCTCTGCAGCAGAAATGACACCGCCTGGGAGGAGCTGAAGCTCACATGTCAAACAGCAATGCA
  CGTCTTGCAGTTAACAATCAAGGAACCGTGGGTTTTATTGGGAGGTGGCTGTACAGAAACACACTTGGCT
  GCATATGTCAGACACAAGGTTCATCACGAGGCAGAAGCTATTGTCAGAGATGATGGGTGTACTCAGGCAA
  AGCTGCATGTTGCTGCTGAAGCATTTTGCAGTGCTCTGGAGTCCGTTGCTGGCTCTTTGGAACATGATGG
  TGGTGAAATCCTCATTGACACGAAGTATGGACACCTTTGGTCCTGTCAAGCAGATTCTGCCTCTGTTGGT
  AACTGGTCAGATACGCTGTCACGGTGTGGCTGTGGTTTGTACAACAGCCAGGAAGAGCTCAGCTGGTCTG
  TCTTAAGAAGTACTTATCATCCTTTTGCACCACAAACCTGCCTTCCACAGGCAGCTTTGGGCTCAGCCAG
  TAACCTGACTGTGGACTGCTTCACTGCCAAGCTGAGTGGCTTACAGGTGGCTGTAGAGACAGCCAATTTG
  ATTTTAGATCTTTCATATGTCATTGAAGATAAAAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286981
• Insert Size: 1509 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286981.1, NP_001273910.1
• RefSeq Size: 3003 bp
• RefSeq ORF: 1509 bp
• Locus ID: 59030
• Cytogenetics: 2 F3
• Gene Summary: This gene encodes a protein which shares sequence similarity with other members of the type II chaperonin family. The encoded protein is a centrosome-shuttling protein and plays an important role in cytokinesis. This protein also interacts with other type II chaperonin members to form a complex known as the BBSome, which involves ciliary membrane biogenesis. This protein is encoded by a downstream open reading frame (dORF). Several upstream open reading frames (uORFs) have been identified, which repress the translation of the dORF, and two of which can encode small mitochondrial membrane proteins. Alternatively spliced transcripts encoding distinct isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (3) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.