Arhgef9 (NM_001290385) Mouse Untagged Clone

CAT#: MC228017

Arhgef9 (untagged) - Mouse CDC42 guanine nucleotide exchange factor (GEF) 9 (Arhgef9), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef9
• Synonyms: 9630036L12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228017 representing NM_001290385
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACCGGGAGTTGGCATTTAAGGCTGGCGACGTCATCAAAGTCTTGGATGCTTCCAACAAGGATT
  GGTGGTGGGGCCAGATCGACGATGAGGAGGGATGGTTTCCTGCCAGCTTTGTGAGGCTCTGGGTGAACCA
  GGAGGATGGGGTGGAGGAAGGGCCCAGTGATGTGCAGAATGGGCACCTGGACCCCAACTCAGACTGCCTT
  TGTCTGGGCCGGCCACTACAGAACCGGGACCAGATGCGGGCCAATGTCATCAATGAGATCATGAGCACGG
  AGCGCCATTACATCAAGCACCTCAAGGACATTTGCGAGGGCTACCTGAAGCAGTGCCGAAAGAGAAGGGA
  CATGTTCAGTGACGAGCAGCTGAAGGTCATCTTTGGAAACATTGAAGACATATACAGATTTCAGATGGGC
  TTCGTGAGAGACCTGGAGAAGCAGTACAACAATGATGATCCCCACCTCAGTGAGATAGGACCCTGCTTCT
  TAGAGCATCAAGATGGATTCTGGATATACTCTGAATATTGTAACAACCACCTGGATGCCTGCATGGAACT
  CTCCAAGCTGATGAAGGACAGCCGCTATCAACACTTCTTTGAGGCCTGCCGCCTCTTGCAGCAGATGATC
  GACATTGCTATTGATGGTTTTCTTTTGACTCCAGTACAGAAGATCTGCAAATATCCCTTACAATTGGCCG
  AGCTCCTAAAGTATACTGCCCAAGATCACAGTGATTACAGATACGTTGCAGCTGCTTTGGCTGTCATGAG
  AAATGTGACTCAGCAGATCAATGAACGCAAGCGACGTCTGGAGAATATTGACAAGATTGCGCAATGGCAG
  GCCTCTGTCCTAGACTGGGAGGGAGAGGACATCTTAGACAGGAGCTCGGAGCTGATCTACACTGGAGAGA
  TGGCCTGGATCTACCAGCCCTATGGCCGCAACCAGCAGAGAGTCTTCTTCCTGTTTGACCACCAGATGGT
  CCTCTGCAAGAAGGACCTAATCCGGAGAGACATCCTATACTACAAGGGGCGCATTGACATGGATAAATAT
  GAGGTAATTGACATCGAAGATGGCAGAGATGATGACTTTAATGTCAGCATGAAAAATGCTTTCAAGCTTC
  ATAACAAGGAAACGGAAGAAGTGCATCTGTTCTTTGCCAAGAAACTGGAGGAGAAAATACGTTGGCTTAG
  AGCTTTCAGAGAAGAGAGAAAAATGGTACAAGAAGATGAAAAAATTGGCTTTGAGATTTCTGAAAATCAG
  AAGAGACAGGCTGCAATGACTGTGAGAAAAGCTTCTAAACAGAAAGGTGTCAACTCTGCCCGCTCAGTTC
  CTCCTTCCTACCCACCACCACAGGACCCGTTAAACCAGGGCCAGTACCTGGTCCCCGATGGCATCGCTCA
  GTCTCAAGTGTTTGAGTTCACTGAACCGAAACGCAGCCAGTCACCATTCTGGCAAAACTTCAGTAGGTTA
  ACCCCCTTCAAAAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290385
• Insert Size: 1488 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290385.1, NP_001277314.1
• RefSeq Size: 5532 bp
• RefSeq ORF: 1488 bp
• Locus ID: 236915
• UniProt ID: Q3UTH8
• Cytogenetics: X C3
• Gene Summary: Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an in-frame downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus than isoform a. Variants 2 and 3 encode the same isoform (b).