Agbl4 (NM_001284190) Mouse Untagged Clone

CAT#: MC228013

Agbl4 (untagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agbl4
• Synonyms: 4930578N11Rik; 4931433A01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228013 representing NM_001284190
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCGGAGCCAGACAGCGCCAGAGGCAGGCAATGATACAGGAAATGAGGATGCCATTGGAGGGA
  ATGTGAACAAATACATAGTGCTTCCAAATGGATACTCTGGACAGCCCAAGAAAGGACATCTTACCTTTGA
  TGCTTGCTTTGAAAGTGGTAACCTCGGCCGGGTTGAGCAAGTCTCTGATTTTGAGTATGATCTGTTCATT
  AGGCCGGACACCTGTAATCCACGCTTCCGAGTCTGGTTCAACTTTACTGTTGAAAATGTGAAAGAATTGC
  AGAGGGTAATTTTCAACATTGTTAACTTCAGTAAAACCAAGAGTCTTTACCGAGATGGGATGGCACCAAT
  GGTGAAATCTACCAGCAGACCAAAATGGCAAAGACTACCACCAAAAAACGTTTATTACTATCGCTGCCCA
  GACCACAGGAAGAACTATGTGATGTCCTTTGCATTCTGTTTTGACCGAGAAGATGATATCTACCAGTTTG
  CTTACTGCTACCCTTATACATATACTCGCTTCCAGCATTACCTCGACAGCTTACAAAAGAAAAACATGGA
  TTATTTCTTCCGGGAACAGCTTGGACAGAGTGTGCAACAGCGGCAGCTTGACCTCCTGACGATAACCAGC
  CCGGAGAATCTCCGTGAAGGGTCAGAAAAGAAGGTGATATTCATCACAGGGCGAGTCCACCCAGGGGAAA
  CGCCATCTTCATTTGTGTGCCAAGGAATCATCGACTTCCTTGTAAGTCAGCATCCAATTGCCCGTGTCCT
  ACGAGAACATTTAGTCTTCAAGATTGCTCCAATGCTCAACCCTGATGGAGTTTACCTGGGCAACTACAGG
  TGTTCCCTGATGGGGTTTGACCTGAATCGTCACTGGCTGGATCCCTCTCCATGGGCCCATCCCACCCTGC
  ATGGAGTGAAACAGCTTATTATCAAGATGTACAATGACCCAAAAACAAGCCTGGAGTTCTATATTGACAT
  CCACGCTCACTCCACCATGATGAATGGCTTCATGTACGGCAATATCTTTGAGGATGAGGAACGGTTCCAA
  AGGCAGTCCATTTTTCCAAAACTCCTTTGCCAGAATGCCGAGGACTTCTCCTATACTAGCACATCCTTCA
  ACAGGGATGCTGTGAAAGCAGGAACTGGCCGGCGCTTCCTGGGCGGGCTTCTGGACCACTCATCATACTG
  CTATACCCTAGAGGTTTCCTTCTACAGCTACATCATTGGGGGTACTACAGCTGCAGTGCCCTACACTGAA
  GAAGCCTATATGAAACTGGGACGGAACGTGGCAAGAACATTTCTAGATTATTACCGGCTGAACTCCCTGG
  TTGAGAAGATAGCAGTCCCCATGCCAAGACTTCGGAAAGAAAAAGCCCCTCCTTGCAAGCACCCACCCCC
  AAGGGGCTCAACCAGCAACGTCTCCATTGGCAAAGGGGACAAGAAGAACTCACTGAACCACAAAGACCCA
  TCAACCCCCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284190
• Insert Size: 1485 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284190.1, NP_001271119.1
• RefSeq Size: 1659 bp
• RefSeq ORF: 1485 bp
• Locus ID: 78933
• Cytogenetics: 4 C7-D1
• Gene Summary: Metallocarboxypeptidase that mediates deglutamylation of target proteins (PubMed:17244818, PubMed:21074048, PubMed:25103237, PubMed:26829768). Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins (PubMed:17244818). Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK (PubMed:21074048). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768). Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 (PubMed:25103237).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate 3' exon structure compared to variant 1. It encodes isoform 3, which is shorter and has a distinct C-terminus, compared to isoform 1.