Celf3 (NM_001289613) Mouse Untagged Clone

CAT#: MC228012

Celf3 (untagged) - Mouse CUGBP, Elav-like family member 3 (Celf3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Celf3
• Synonyms: 4930415M08Rik; BRUNOL1; CAGH4; ERDA4; Tnrc4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228012 representing NM_001289613
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGCCAGATGCCATCAAGCTGTTTGTGGGGCAGATCCCGAGGCATCTGGAGGAAAAGGACCTGA
  AGCCCATCTTCGAGCAGTTTGGTCGGATCTTCGAGCTGACTGTCATCAAGGACAAGTACACCGGGCTGCA
  CAAGGGATGTGCTTTCCTGACGTACTGTGCTCGCGATTCAGCCCTGAAGGCCCAGAGTGCCCTGCACGAA
  CAGAAGACTCTCCCAGGGATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGTCGAGGAGAAG
  ACCGGAAGCTCTTTGTGGGCATGCTAGGAAAGCAGCAGACAGATGAGGATGTCCGGAAGATGTTTGAACC
  ATTTGGGACTATAGACGAGTGCACTGTGCTCCGGGGGCCAGACGGTACCAGCAAAGGCTGTGCCTTTGTG
  AAGTTCCAGACTCACGCTGAGGCCCAGGCAGCCATCAACACCCTTCACAGCAGCCGGACCCTACCGGGTG
  CCTCATCCAGCCTGGTGGTAAAGTTTGCTGACACGGAGAAGGAGCGAGGTCTCCGTCGAATGCAGCAGGT
  GGCTACCCAGCTGGGCATGTTCAGCCCGATCGCCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCC
  CTGATGCAGCAGCAGGCGGCCCTGGTAGCAGCTCACAGTGCCTACCTCAGCCCTATGGCCACCATGGCTG
  CCGTGCAGATGCAGCACATGGCTGCCATCAGTGCCAATGGCCTCATCGCCACCCCCATCACTCCATCCTC
  AGGAACCAGCACCCCTCCTGCCATTGCTGCCACGCCCGTCTCTGCCATCCCTGCTGCCTTGGGCGTCAAC
  GGCTACAGCCCGGTGCCCACCCAGCCTACAGGGCAGCCTGCCCCGGATGCTCTGTATCCCAACGGGGTTC
  ACCCTTACCCAGATGAGGCTCTGTCTGCTGAGAGAAGTGCTGGCGGGGTTCCCATAATGTCCCAGGCCCA
  CTCATGGCTTGTGATGCTCTCTGCAGCCCAGAGCCCGGCAGCCCCCGTGGACCCTCTCCAGCAGGCCTAT
  GCAGGAATGCAGCACTACACAGCAGCGTACCCCGCAGCCTACAGCCTGGTTGCACCTGCGTTCCCGCAGC
  CTCCAGCCCTGGTTGCCCAGCAGCCCCCACCACCACCTCAGCAACAGCAGCAGCAGCAGCAGCAGCAACA
  GCAACAGCAGCAGCAACGGGAAGGCCCTGATGGCTGCAACATCTTCATCTACCACCTGCCCCAGGAGTTC
  ACAGACTCAGAGATCCTCCAGATGTTTGTCCCTTTTGGTCATGTCATCTCAGCCAAAGTCTTTGTTGACC
  GGGCCACCAATCAGAGCAAATGTTTTGGCTTTGTGAGTTTCGACAATCCGGCCAGTGCCCAGGCTGCCAT
  CCAGGCTATGAACGGTTTCCAGATTGGCATGAAGCGCCTCAAAGTCCAGCTAAAGCGGCCTAAGGATGCA
  AACAGGCCCTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289613
• Insert Size: 1485 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289613.1, NP_001276542.1
• RefSeq Size: 3136 bp
• RefSeq ORF: 1485 bp
• Locus ID: 78784
• UniProt ID: Q8CIN6
• Cytogenetics: 3 F2.1
• Gene Summary: RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]