Hsf4 (NM_001256042) Mouse Untagged Clone

CAT#: MC228002

Hsf4 (untagged) - Mouse heat shock transcription factor 4 (Hsf4), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsf4
• Synonyms: HSF 4; HSTF 4; HSTF4; ldis1; mHSF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228002 representing NM_001256042
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAAGCGCCAGCTGCGCTGCCCACGGAGCCAGGCCCCAGCCCGGTACCTGCCTTCCTCGGCAAGC
  TATGGGCGCTGGTAGGCGACCCAGGCACCGACCACCTCATCCGCTGGAGCCCGAGTGGCACCAGCTTCCT
  CGTAAGTGATCAGAGCCGCTTCGCCAAGGAAGTGCTGCCCCAGTACTTCAAGCACAGCAACATGGCGAGC
  TTTGTTCGCCAACTCAACATGTATGGTTTTCGGAAGGTGGTGAGCATCGAGCAAGGTGGCCTGCTCAGAC
  CAGAGCGTGACCACGTTGAGTTTCAGCATCCGAGCTTCGTGCGAGGCCGCGAGCAGCTACTGGAGCGCGT
  GCGCCGCAAGGTACCTGCGCTGCGAGGCGATGACAGTCGATGGCGTCCGGAAGACCTGAGCCGACTGCTG
  GGAGAGGTGCAAGCTTTGAGAGGAGTGCAGGAGAGCACGGAGGCACGGCTGCAGGAACTCAGGCAGCAGA
  ACGAGATCTTGTGGCGAGAGGTGGTAACTTTGCGGCAGAGCCACAGTCAGCAGCACCGGGTCATCGGCAA
  GCTAATCCAGTGCCTGTTTGGGCCACTTCAGACAGGGCCCAGCAGTACAGGAGCCAAGAGAAAACTGTCC
  CTAATGCTAGATGAGGGGAGCGCCTGTTCAGCATCTGCCAAATTCAATGCCTGCCCTGTGTCTGGTGCTC
  TCCTCCAGGACCCCTACTTTATCCAGTCGCCCCTCCCAGAGACCACGTTGGGCCTCAGCCCTCACAGGGC
  CAGAGGGCCCATCATCTCTGACATCCCAGAAGATTCTCCATCTCCTGAAGGACACAGGCTTTCTCCCTCC
  GGTGGTTGCAGGAGGGTGAAGGGCCTGGCACTGCTCAAAGAAGAGCCGGCCAGTCCAGGGGGGGATGGCG
  AGGCCGGGCTGGCCCTGGCCCCAAACGAGTGTGACTTCTGCGTGACAGCACCCCCACCGCTGCCCGTGGC
  TGTGGTGCAGGCCATCCTGGAAGGGAAAGGGAGCTACAGCCCTGAGGGGCCCAGGAGTGTACAACAGCCT
  GAACCAAGGGGCCCCAGGGAGGTACCTGACAGGGGAACTCTGGGCCTGGATCGGGGTAACCGGAGCCCAG
  AGAGTCTGCTACCCCCAATGCTGCTTCGGCCTGCCCCTGAAACTCTGGAGCCCGTGGCACCTGTGGATGT
  GCTCGGCCCTAGCCTGCATGGACGAGAATGGACCTTGATGGATCTGGACATGGAGTTGTCTCTGATGCAG
  CCCTTGGCACCAGAGACGGATGAAGCAGAGCTGACGGTCAAGGAGTTGAATTCTTCTGGTGTAGGGAAAG
  ACCACACACTGGGAACTCCACTCATGCTAGATGTCCAGGCGGATTTGGAGGGTGCAGCCCTGTCTGTGCC
  TGGGGCTTTAACCCTGTACAACGTCACCGAGAGCAACGCCTCCTACTTGGATCCTGGAGCCAGTCCCTCC
  TCTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256042
• Insert Size: 1479 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256042.1, NP_001242971.1
• RefSeq Size: 1739 bp
• RefSeq ORF: 1479 bp
• Locus ID: 26386
• UniProt ID: Q9R0L1
• Cytogenetics: 8 D3
• Gene Summary: DNA-binding protein that specifically binds heat shock promoter elements (HSE). The HSF4A isoform represses transcription while the HSF4B isoform activates transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.