Mtf2 (NM_001253880) Mouse Untagged Clone

CAT#: MC227994

Mtf2 (untagged) - Mouse metal response element binding transcription factor 2 (Mtf2), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mtf2
• Synonyms: 9230112N11Rik; AA537621; C76717; M96; Pcl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227994 representing NM_001253880
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGTACAATATGTCAAGAAGAGTATTCAGAAGCTCCCAATGAAATGGTTATATGTGATAAGTGTG
  GTCAAGGATATCATCAGCTGTGTCACACACCTCATATTGACTCGAGTGTGATTGATTCAGATGAAAAGTG
  GCTTTGTCGACAGTGCGTTTTTGCAACAACTACAAAGAGGGGTGGTGCGCTTAAGAAAGGACCAAATGCC
  AAAGCATTGCAAGTCATGAAGCAGACATTACCCTATAGTGTGGCTGACCTTGAATGGGATGCAGGCCATA
  AAACCAATGTCCAGCAGTGCTACTGCTATTGTGGAGGCCCTGGAGACTGGTATTTAAAGATGCTGCAGTG
  CTGCAAATGTAAGCAGTGGTTTCATGAAGCTTGTGTCCAATGCCTTCAGAAGCCAATGCTGTTTGGAGAT
  AGGTTTTATACATTTATTTGCTCTGTCTGCAGTTCTGGACCAGAATACCTCAAACGTCTACCGTTACAGT
  GGGTAGATATAGCACACCTATGCCTTTACAACCTAAGTGTTATTCACAAGAAGAAATACTTTGATTCTGA
  ACTTGAGCTTATGACATACATTAATGAAAACTGGGATAGATTGCACCCTGGAGAGCTGGCAGACACACCC
  AAATCTGAAAGATATGAGCATGTTCTGGAGGCATTAAATGATTACAAGACCATGTTTATGTCTGGGAAAG
  AAATAAAGAAGAAGAAGCATTTGTTTGGGTTGCGAATTCGTGTTCCTCCTGTGCCACCAAATGTGGCTTT
  CAAAGCAGAGAAAGAACCAGAAGGAACATCCCATGAATTTAAAATTAAAGGCAGAAAGGCATCCAAACCT
  ACATCTGACTCAAGGGAAGTAAGCAATGGGATAGAAAAAAAAGGAAAGAAAAAATCTGTAGGTCGTCCTC
  CTGGCCCATATACAAGAAAAATGATTCAAAAAACTGCTGAGCTACCTTTGGATAAGGAATCAGTTTCAGA
  GAATCCTACGTTGGATTTACCTTGTTCTATAGGGAGAACTGAGGGAATTGCACATTCATCCAATACCTCA
  GATGTGGACCTCACGGGTGCTTCCAGTGCAAACGAAACTACCTCGGCTAGCATTTCCAGGCATTGTGGAT
  TATCAGACTCTAGAAAAAGAACTCGCACAGGAAGATCATGGCCTGCTGCAATACCACATTTGCGGAGAAG
  AAGAGGTCGCCTTCCACGAAGAGCACTCCAGACTCAGAACTCAGAAGTTGTGAAAGATGATGAAGGCAAA
  GAAGATTATCAGTTTGAAGAACTTAACACAGAAATTCTGAATAACTTAGCAGATCAGGAGTTGCAGCTCA
  ATCACCTGAAAAACTCCATCACTAGTTATTTTGGTGCCGCAGGTAGAATAGCATGTGGCGAAAAATACAG
  AGTTTTGGCTCGTCGGGTGACGCTTGATGGGAAGGTGCAGTATCTTGTGGAATGGGAAGGAGCGACTGCA
  TCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253880
• Insert Size: 1476 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253880.1, NP_001240809.1
• RefSeq Size: 4062 bp
• RefSeq ORF: 1476 bp
• Locus ID: 17765
• UniProt ID: Q02395
• Cytogenetics: 5 F
• Gene Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 7, which results in the use of a downstream AUG start codon. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3. Variants 2-6 all encode the same isoform (2).