Rabgef1 (NM_001199059) Mouse Untagged Clone

CAT#: MC227983

Rabgef1 (untagged) - Mouse RAB guanine nucleotide exchange factor (GEF) 1 (Rabgef1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rabgef1
• Synonyms: Rab5ef; Rabex5; Rin2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227983 representing NM_001199059
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTGAAGTCCGAACGCAGGGGAATTCATGTGGATCAATCTGAGCTCCTGTGCAAGAAAGGATGCG
  GTTACTACGGCAACCCTGCCTGGCAGGGTTTCTGCTCCAAGTGCTGGAGGGAGGAGTACCACAAGGCCCG
  GCAGAGGCAGATCCAAGAGGACTGGGAACTGGCAGAACGACTTCAGCGGGAGGAGGAAGAGGCCTTCGCG
  AGCAGCCAGAGCAGCCAAGGAGCCCAGTCCCTCACCTTCTCCAAGTTCGAGGAGAAGAAGACCAATGAGA
  AAACCCGAAAAGTCACCACAGTGAAGAAGTTCTTCAGCGCCTCTTCCAGAGCTGGATCCAAGAAGGAAAT
  TCAGGAAGCCAAAGCTCCCAGTCCCTCCATAAACCGGCAAACCAGCATTGAGACGGACCGAGTGACTAAG
  GAGTTCATAGACTTTCTCAAGACCTTCCACAAGACAGGCCAAGAAGTCTATAAACAGACGAAGATGTTTT
  TGGAAGCAATGCCTTATAAAAGGGATTTAAGCATCGAGGAACAGTCAGAATGTACTCAGGACTTTTACCA
  AAATGTGGCTGAAAGAATGCAGACCCGTGGGAAAGTGCCTCCAGAGAAAGTGGAGAAGATAATGGATCAG
  ATCGAAAAGCACATCATGACGCGTCTCTATAAATTTGTGTTCTGCCCAGAGACTACTGATGATGAGAAGA
  AAGATCTCGCCATTCAAAAAAGAATCAGGGCCCTGCACTGGGTAACGCCTCAGATGCTCTGTGTCCCTGT
  CAATGAGGAAATCCCTGAAGTGTCCGACATGGTGGTGAAAGCGATCACAGACATCATTGAGATGGACTCA
  AAGCGTGTGCCTCGGGACAAGCTGGCCTGCATCACCAGGTGCAGCAAGCACATCTTCAATGCCATCAAGA
  TCACCAAGAATGAGCCAGCCTCTGCCGATGACTTCCTGCCCACCCTGATCTACATCGTCCTGAAGGGCAA
  CCCCCCTCGCCTGCAGTCCAACATCCAGTACATCACTCGCTTCTGCAACCCCAGCCGGCTCATGACGGGC
  GAGGATGGCTACTACTTCACCAACCTGTGCTGTGCTGTGGCTTTCATTGAGAAATTAGACGCCCAGTCTT
  TGAATTTAAGTCAGGAGGATTTTGACCGGTACATGTCTGGCCAGACATCCCCCAGGAAGCAGGAGTCTGA
  GAGTTGGCCCCCGGAGGCCTGCTTAGGTGTGAAGCAAATGTATAAGAACTTGGACCTCCTGTCTCAGTTG
  AATGAACGGCAAGAAAGGATCATGAACGAAGCCAAGAAACTTGAAAAAGACTTAATAGACTGGACAGACG
  GGATTGCCAAGGAAGTTCAAGACATTGTTGAGAAATACCCACTGGAGATTAAGCCCCCGAACCAACCCTT
  AGCAGCCATCGACTCTGAGAATGTGGAGAACGACAAGCTCCCTCCCCCTCTGCAGCCTCAGGTGTACGCA
  GGGTGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_001199059
• Insert Size: 1476 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199059.1, NP_001185988.1
• RefSeq Size: 2698 bp
• RefSeq ORF: 1476 bp
• Locus ID: 56715
• UniProt ID: Q9JM13
• Cytogenetics: 5 G1.3
• Gene Summary: Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A (By similarity). Can act as a ubiquitin ligase (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).